PR1-mediated defence via C-terminal peptide release is targeted by a fungal pathogen effector
Date
2021
Authors
Sung, Yi-Chang
Outram, Megan
Breen, Susan
"0000-0002-0508-0084"
Dagvadorj, Bayantes
Winterberg, Britta
Kobe, Bostjan
Williams, Simon
Solomon, Peter
Journal Title
Journal ISSN
Volume Title
Publisher
Wiley
Abstract
The effector SnTox3 from Parastagonospora nodorum elicits a strong necrotic response in susceptible wheat and also interacts with wheat pathogenesis-related protein 1 (TaPR-1), although the function of this interaction in disease is unclear. Here, we dissect TaPR1 function by studying SnTox3-TaPR1 interaction and demonstrate the dual functionality of SnTox3.
We utilized site-directed mutagenesis to identify an SnTox3 variant, SnTox3(P173S), that was unable to interact with TaPR1 in yeast-two-hybrid assays. Additionally, using recombinant proteins we established a novel protein-mediated phenotyping assay allowing functional studies to be undertaken in wheat.
Wheat leaves infiltrated with TaPR1 proteins showed significantly less disease compared to control leaves, correlating with a strong increase in defence gene expression. This activity was dependent on release of the TaCAPE1 peptide embedded within TaPR1 by an unidentified serine protease. The priming activity of TaPR1 was compromised by SnTox3 but not the noninteracting variant SnTox3(P173S), and we demonstrate that SnTox3 prevents TaCAPE1 release from TaPR1 in vitro.
SnTox3 independently functions to induce necrosis through recognition by Snn3 and also suppresses host defence through a direct interaction with TaPR1 proteins. Importantly, this study also advances our understanding of the role of PR1 proteins in host-microbe interactions as inducers of host defence signalling.
Description
Keywords
CAPE1, effector-triggeredsusceptibility, Parastagonospora nodorum, pathogenesis-related 1 (PR-1), Septorianodorum blotch, SnTox3
Citation
Collections
Source
New Phytologist
Type
Journal article
Book Title
Entity type
Access Statement
License Rights
Restricted until
2099-12-31
Downloads
File
Description