Positive selection in AvrP4 avirulence gene homologues across the genus Melampsora

dc.contributor.authorVan der Merwe, Marlien
dc.contributor.authorKinnear, Mark
dc.contributor.authorBarrett, Luke
dc.contributor.authorDodds, Peter N
dc.contributor.authorEricson, Lars
dc.contributor.authorThrall, P
dc.contributor.authorBurdon, Jeremy J.
dc.date.accessioned2015-12-10T22:55:08Z
dc.date.issued2009
dc.date.updated2016-02-24T12:06:30Z
dc.description.abstractPathogen genes involved in interactions with their plant hosts are expected to evolve under positive Darwinian selection or balancing selection. In this study a single copy avirulence gene, AvrP4, in the plant pathogen Melampsora lini, was used to investigate the evolution of such a gene across species. Partial translation elongation factor 1-alpha sequences were obtained to establish phylogenetic relationships among the Melampsora species. We amplified AvrP4 homologues from species pathogenic on hosts from different plant families and orders, across the inferred phylogeny. Translations of the AvrP4 sequences revealed a predicted signal peptide and towards the C-terminus of the protein, six identically spaced cysteines were identified in all sequences. Maximum likelihood analysis of synonymous versus nonsynonymous substitution rates indicated that positive selection played a role in the evolution of the gene during the diversification of the genus. Fourteen codons under significant positive selection reside in the C-terminal 28 amino acid region, suggesting that this region interacts with host molecules in most sequenced accessions. Selection pressures on the gene may be either due to the pathogenicity or avirulence function of the gene or both.
dc.identifier.issn0962-8452
dc.identifier.urihttp://hdl.handle.net/1885/59970
dc.publisherRoyal Society of London
dc.sourceProceedings of the Royal Society of London Series B: Biological Sciences
dc.subjectKeywords: signal peptide; fungus; gene expression; peptide; phylogeny; plant-pollinator interaction; rust disease; species diversity; virulence; article; avrp4 gene; fungal gene; fungal genetics; fungal virulence; fungus; genetic selection; Melampsora; molecular ev Avirulence; Effector; Melampsora; Pathogen; Rust fungi; Selection
dc.titlePositive selection in AvrP4 avirulence gene homologues across the genus Melampsora
dc.typeJournal article
local.bibliographicCitation.lastpage2922
local.bibliographicCitation.startpage2913
local.contributor.affiliationVan der Merwe, Marlien, CSIRO
local.contributor.affiliationKinnear, Mark, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationBarrett, Luke, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationDodds, Peter N, CSIRO
local.contributor.affiliationEricson, Lars, Umea University
local.contributor.affiliationThrall, P, CSIRO
local.contributor.affiliationBurdon, Jeremy J., CSIRO
local.contributor.authoremailrepository.admin@anu.edu.au
local.contributor.authoruidKinnear, Mark, u4340699
local.contributor.authoruidBarrett, Luke, u4089759
local.description.embargo2037-12-31
local.description.notesImported from ARIES
local.identifier.absfor060307 - Host-Parasite Interactions
local.identifier.ariespublicationu9511635xPUB515
local.identifier.citationvolume276
local.identifier.doi10.1098/rspb.2009.0328
local.identifier.scopusID2-s2.0-68249085537
local.identifier.uidSubmittedByu9511635
local.type.statusPublished Version

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