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Hydrolysis of the 5'- p -nitrophenyl ester of TMP by oligoribonucleases (ORN) from Escherichia coli , Mycobacterium smegmatis , and human

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Date

2008

Authors

Park, Ah Young
Elvin, Christopher
Hamdan, Samir
Wood, Robert
Liyou, Nancy
Hamwood, Tamarind E
Jennings, Phil A
Dixon, Nicholas

Journal Title

Journal ISSN

Volume Title

Publisher

Academic Press

Abstract

Escherichia coli oligoribonuclease (EcoORN), encoded by the orn gene, is a 3′-5′ exonuclease that degrades short single-stranded oligoribonucleotides to rNMPs in the final step of RNA degradation. The orn gene is essential in E. coli, but not in highe

Description

Keywords

Keywords: Bacteria (microorganisms); Escherichia coli; Gammaproteobacteria; Mycobacterium smegmatis; exoribonuclease; His His His His His His; His-His-His-His-His-His; histidine; hybrid protein; nitrophenol; oligopeptide; oligoribonuclease; protein subunit; thymidi 3'-5' exonuclease; DEDDh; Oligoribonuclease; RNA degradation; Spectrophotometric assay

Citation

URI

http://hdl.handle.net/1885/49841

Collections

ANU Research Publications

Source

Protein Expression and Purification

Type

Journal article

Book Title

Entity type

Access Statement

License Rights

DOI

10.1016/j.pep.2007.10.005

Restricted until

2037-12-31

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01_Park_Hydrolysis_of_the_5'-_p_2008.pdf (633.67 KB)

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