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Isolation and characterization of 15 polymorphic microsatellites in the Plethodontid salamander Ensatina eschscholtzii

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dc.contributor.authorDevitt, Thomas J.
dc.contributor.authorPereira, Ricardo
dc.contributor.authorJakkula, Lakshmi
dc.contributor.authorAlexandrino, Joao
dc.contributor.authorBardeleben, Carolyne
dc.contributor.authorMoritz, Craig
dc.date.accessioned2015-12-13T22:45:38Z
dc.date.issued2009
dc.date.updated2016-02-24T09:41:06Z
dc.description.abstractWe developed 15 new polymorphic microsatellites for the plethodontid salamander Ensatina eschscholtzii. Loci were isolated from a genomic library from Ensatina eschscholtzii xanthoptica enriched for (AAAG)n repetitive elements. The number of alleles per locus ranged from 4 to 20 (mean 9) in the sampled population. Observed heterozygosity ranged from 0.37 to 1. None of the loci deviated from Hardy-Weinberg equilibrium or showed significant linkage disequilibrium after a Bonferroni correction for multiple comparisons. All loci amplified in the six other subspecies of the Ensatina eschscholtzii complex. These new markers will prove useful in measuring gene flow and population structure as well as patterns of mating and sperm use in Ensatina.
dc.identifier.issn1755-098X
dc.identifier.urihttp://hdl.handle.net/1885/79883
dc.publisherWiley-Blackwell
dc.sourceMolecular Ecology Resources
dc.subjectKeywords: Ensatina; Ensatina eschscholtzii; Ensatina eschscholtzii xanthoptica; Plethodontidae; Salamandroidea Ensatina; Microsatellite; Plethodontid; Plethodontidae; Ring species; Salamander
dc.titleIsolation and characterization of 15 polymorphic microsatellites in the Plethodontid salamander Ensatina eschscholtzii
dc.typeJournal article
local.bibliographicCitation.issue3
local.bibliographicCitation.lastpage969
local.bibliographicCitation.startpage966
local.contributor.affiliationDevitt, Thomas J., University of California
local.contributor.affiliationPereira, Ricardo, Universidade de Porto
local.contributor.affiliationJakkula, Lakshmi, Lawrence Berkeley National Laboratory
local.contributor.affiliationAlexandrino, Joao, Universidade Estadual Paulista
local.contributor.affiliationBardeleben, Carolyne, University of California
local.contributor.affiliationMoritz, Craig, College of Medicine, Biology and Environment, ANU
local.contributor.authoruidMoritz, Craig, u1572787
local.description.embargo2037-12-31
local.description.notesImported from ARIES
local.identifier.absfor060306 - Evolutionary Impacts of Climate Change
local.identifier.absfor060302 - Biogeography and Phylogeography
local.identifier.absfor050202 - Conservation and Biodiversity
local.identifier.ariespublicationf5625xPUB8247
local.identifier.citationvolume9
local.identifier.doi10.1111/j.1755-0998.2009.02518.x
local.identifier.scopusID2-s2.0-65249168756
local.identifier.thomsonID000265897200083
local.type.statusPublished Version

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