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Connecting Mammalian Genome with Phenome by ENU Mouse Mutagenesis: Gene Combinations Specifying the Immune System

Date

2005

Authors

Papathanasiou, Panagiotis
Goodnow, Christopher

Journal Title

Journal ISSN

Volume Title

Publisher

Annual Reviews Inc

Abstract

The human and mouse genome sequences bring closer the goal of understanding how characteristics of adult mammalian physiology and pathology are encoded by DNA. Here we review the challenge of understanding how genes specify mammalian traits, with particular focus on the cells and behavior of the immune system. Summarized is the emerging experience, advantages, and limitations of using ethylnitrosourea (ENU) to modify the mouse genome and select informative variants by phenotypic screens, yielding two main conclusions. First, ENU-induced variation provides an eminently feasible route to understanding how the genome encodes important mammalian processes without any prior assumptions about genes, their chromosomal locations, or expression patterns. Second, ENU alleles match those arising by natural variation. By changing individual protein domains or splice products, these alleles reveal separate gene functions specified through protein combinations.

Description

Keywords

Keywords: ethylnitrosourea; allelism; gene expression; gene frequency; gene function; gene location; gene sequence; genetic variability; genome; immune system; innate immunity; mouse; mutagenesis; nonhuman; phenotype; priority journal; review; RNA splicing; T lymph Allele; Genome; Mouse; Mutagenesis; Phenome; T lymphocyte

Citation

Source

Annual Review of Genetics

Type

Journal article

Book Title

Entity type

Access Statement

License Rights

DOI

10.1146/annurev.genet.39.110304.095817

Restricted until

2037-12-31