SWET for secure water suppression on probes with high quality factor

Date

2005

Authors

Wu, Peter
Otting, Gottfried

Journal Title

Journal ISSN

Volume Title

Publisher

Kluwer Academic Publishers

Abstract

Water suppression by selective preirradiation is increasingly difficult to achieve on probeheads with high quality factor because of the opposing forces of radiation damping. Here we show that a simple modification to the WET scheme provides reliable water suppression in aqueous solutions of proteins and peptides with minimal saturation of the Hα protons. The scheme is shown to work also with dilute peptide solutions. It is recommended to maintain the water suppression during the evolution time of COSY experiments by weak selective irradiation that causes only minimal Bloch-Siegert shifts. The new water-suppression scheme suppresses the water magnetization by spatial scrambling. Traditional water suppression by preirradiation is similarly based more on water scrambling due to the radiofrequency inhomogeneity than on relaxation effects.

Description

Keywords

Keywords: water; aqueous solution; article; Fourier transformation; image processing; image quality; molecular probe; nuclear magnetic resonance imaging; oxygen saturation; priority journal; proton nuclear magnetic resonance; proton radiation; proton transport; rad COSY; Preirradiation; Radiation damping; Water suppression; WET

Citation

Source

Journal of Biomolecular NMR

Type

Journal article

Book Title

Entity type

Access Statement

License Rights

DOI

10.1007/s10858-005-8531-6

Restricted until

2037-12-31