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Adherens junction remodelling during mitotic rounding of pseudostratified epithelial cells

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dc.contributor.authorAguilar‐Aragon, Mario
dc.contributor.authorBonello, Teresa
dc.contributor.authorBell, Graham P.
dc.contributor.authorFletcher, Georgina
dc.contributor.authorThompson, Barry
dc.date.accessioned2020-09-23T01:30:25Z
dc.date.available2020-09-23T01:30:25Z
dc.date.issued2020
dc.date.updated2020-06-23T00:57:52Z
dc.description.abstractEpithelial cells undergo cortical rounding at the onset of mitosis to enable spindle orientation in the plane of the epithelium. In cuboidal epithelia in culture, the adherens junction protein Ecadherin recruits Pins/LGN/GPSM2 and Mud/NuMA to orient the mitotic spindle. In the pseudostratified columnar epithelial cells of Drosophila, septate junctions recruit Mud/NuMA to orient the spindle, while Pins/LGN/GPSM2 is surprisingly dispensable. We show that these pseudostratified epithelial cells downregulate Ecadherin as they round up for mitosis. Preventing cortical rounding by inhibiting Rho-kinase-mediated actomyosin contractility blocks downregulation of E-cadherin during mitosis. Mitotic activation of Rho-kinase depends on the RhoGEF ECT2/Pebble and its binding partners RacGAP1/MgcRacGAP/CYK4/Tum and MKLP1/KIF23/ZEN4/ Pav. Cell cycle control of these Rho activators is mediated by the Aurora A and B kinases, which act redundantly during mitotic rounding. Thus, in Drosophila pseudostratified epithelia, disruption of adherens junctions during mitosis necessitates planar spindle orientation by septate junctions to maintain epithelial integrity.en_AU
dc.description.sponsorshipThis work was funded by the Francis Crick Institute (FC0010180), which is a joint initiative of Cancer Research UK (FC0010180), the Medical Research Council (FC001180), and Wellcome (FC001180). This work was also funded by The Australian National Universityen_AU
dc.format.mimetypeapplication/pdfen_AU
dc.identifier.issn1469-221Xen_AU
dc.identifier.urihttp://hdl.handle.net/1885/211368
dc.language.isoen_AUen_AU
dc.provenance© 2020 The Authors. Published under the terms of the CC BY 4.0 license. This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en_AU
dc.publisherEMBO Pressen_AU
dc.rights© 2020 The Authorsen_AU
dc.rights.licenseCC BY 4.0 licenseen_AU
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_AU
dc.sourceEMBO Reportsen_AU
dc.titleAdherens junction remodelling during mitotic rounding of pseudostratified epithelial cellsen_AU
dc.typeJournal articleen_AU
dcterms.accessRightsOpen Accessen_AU
local.bibliographicCitation.issue4en_AU
local.bibliographicCitation.lastpage16en_AU
local.bibliographicCitation.startpage1en_AU
local.contributor.affiliationAguilar‐Aragon, Mario, Francis Crick Instituteen_AU
local.contributor.affiliationBonello, Teresa, College of Health and Medicine, ANUen_AU
local.contributor.affiliationBell, Graham P., Francis Crick Instituteen_AU
local.contributor.affiliationFletcher, Georgina, The Francis Crick Instituteen_AU
local.contributor.affiliationThompson, Barry, College of Health and Medicine, ANUen_AU
local.contributor.authoruidBonello, Teresa, u1088234en_AU
local.contributor.authoruidThompson, Barry, u1058407en_AU
local.description.notesImported from ARIESen_AU
local.identifier.absfor111201 - Cancer Cell Biologyen_AU
local.identifier.absfor060103 - Cell Development, Proliferation and Deathen_AU
local.identifier.absfor060403 - Developmental Genetics (incl. Sex Determination)en_AU
local.identifier.absseo920102 - Cancer and Related Disordersen_AU
local.identifier.ariespublicationu6269649xPUB395en_AU
local.identifier.citationvolume21en_AU
local.identifier.doi10.15252/embr.201949700en_AU
local.identifier.scopusID2-s2.0-85079151205
local.publisher.urlhttp://embor.embopress.org/en_AU
local.type.statusPublished Versionen_AU

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