Probing the closed-loop model of mRNA translation in living cells

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dc.contributor.authorArcher, Stuart K.
dc.contributor.authorShirokikh, Nikolay E.
dc.contributor.authorHallwirth, Claus V.
dc.contributor.authorBeilharz, Traude H.
dc.contributor.authorPreiss, Thomas
dc.date.accessioned2015-09-07T02:35:56Z
dc.date.available2015-09-07T02:35:56Z
dc.date.issued2015-03-31
dc.description.abstractThe mRNA closed-loop, formed through interactions between the cap structure, poly(A) tail, eIF4E, eIF4G and PAB, features centrally in models of eukaryotic translation initiation, although direct support for its existence in vivo is not well established. Here, we investigated the closed-loop using a combination of mRNP isolation from rapidly cross-linked cells and high-throughput qPCR. Using the interaction between these factors and the opposing ends of mRNAs as a proxy for the closed-loop, we provide evidence that it is prevalent for eIF4E/4G-bound but unexpectedly sparse for PAB1-bound mRNAs, suggesting it primarily occurs during a distinct phase of polysome assembly. We observed mRNA-specific variation in the extent of closed-loop formation, consistent with a role for polysome topology in the control of gene expression.en_AU
dc.description.sponsorshipFunding was provided by the Australian Research Council under grant number DP1300101928. TP was supported by an NHMRC Senior Research Fellowship. NES was supported by a Go8 European Fellowship.en_AU
dc.identifier.issn1547-6286en_AU
dc.identifier.urihttp://hdl.handle.net/1885/15230
dc.publisherTaylor & Francisen_AU
dc.relationhttp://purl.org/au-research/grants/arc/DP1300101928en_AU
dc.rights© Stuart K Archer, Nikolay E Shirokikh, Claus V Hallwirth, Traude H Beilharz, and Thomas Preiss. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.en_AU
dc.sourceRNA Biologyen_AU
dc.subjectcap-poly(a) synergyen_AU
dc.subjecteukaryotic translationen_AU
dc.subjectmrna closed-loopen_AU
dc.subjectpolysomes, ribosomal recyclingen_AU
dc.titleProbing the closed-loop model of mRNA translation in living cellsen_AU
dc.typeJournal articleen_AU
dcterms.accessRightsOpen Access
local.bibliographicCitation.issue3en_AU
local.bibliographicCitation.lastpage254en_AU
local.bibliographicCitation.startpage248en_AU
local.contributor.affiliationArcher, S. K., The John Curtin School of Medical Research, The Australian National Universityen_AU
local.contributor.affiliationPreiss, T., The John Curtin School of Medical Research, The Australian National Universityen_AU
local.contributor.authoremailstuart.archer@anu.edu.auen_AU
local.contributor.authoruidu4000764en_AU
local.identifier.citationvolume12en_AU
local.identifier.doi10.1080/15476286.2015.1017242en_AU
local.identifier.essn1555-8584en_AU
local.identifier.uidSubmittedByu1005913en_AU
local.publisher.urlhttp://www.routledge.com/en_AU
local.type.statusPublished Versionen_AU

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