Initiation of simple and complex spikes in cerebellar Purkinje cells

dc.contributor.authorPalmer, Lucy
dc.contributor.authorClark, Beverley A
dc.contributor.authorGrundemann, Jan
dc.contributor.authorRoth, Arnd
dc.contributor.authorStuart, Gregory J
dc.contributor.authorHausser, Michael
dc.date.accessioned2015-12-07T22:17:14Z
dc.date.issued2010
dc.date.updated2016-02-24T11:26:27Z
dc.description.abstractCerebellar Purkinje cells produce two distinct forms of action potential output: simple and complex spikes. Simple spikes occur spontaneously or are driven by parallel fibre input, while complex spikes are activated by climbing fibre input. Previous studies indicate that both simple and complex spikes originate in the axon of Purkinje cells, but the precise location where they are initiated is unclear. Here we address where in the axon of cerebellar Purkinje cells simple and complex spikes are generated. Using extracellular recording and voltage-sensitive dye imaging in rat and mouse Purkinje cells, we show that both simple and complex spikes are generated in the proximal axon, ∼15-20 μm from the soma. Once initiated, simple and complex spikes propagate both down the axon and back into the soma. The speed of backpropagation into the soma was significantly faster for complex compared to simple spikes, presumably due to charging of the somatodendritic membrane capacitance during the climbing fibre synaptic conductance. In conclusion, we show using two independent methods that the final integration site of simple and complex spikes is in the proximal axon of cerebellar Purkinje cells, at a location corresponding to the distal end of the axon initial segment.
dc.identifier.issn0022-3751
dc.identifier.urihttp://hdl.handle.net/1885/18444
dc.publisherCambridge University Press
dc.sourceJournal of Physiology
dc.subjectKeywords: dye; action potential; animal cell; article; cerebellum; molecular imaging; mouse; nerve fiber; nerve fiber membrane conductance; nerve fiber membrane potential; nonhuman; priority journal; Purkinje cell; rat; recording; synaptic potential; Action Potenti
dc.titleInitiation of simple and complex spikes in cerebellar Purkinje cells
dc.typeJournal article
local.bibliographicCitation.issue10
local.bibliographicCitation.lastpage1717
local.bibliographicCitation.startpage1709
local.contributor.affiliationPalmer, Lucy, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationClark, Beverley A, Wolfson Institute
local.contributor.affiliationGrundemann, Jan, Wolfson Institute London
local.contributor.affiliationRoth, Arnd, Wolfson Institute London
local.contributor.affiliationStuart, Gregory J, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHausser, Michael, University College London
local.contributor.authoremailu8807467@anu.edu.au
local.contributor.authoruidPalmer, Lucy, u4089874
local.contributor.authoruidStuart, Gregory J, u8807467
local.description.embargo2037-12-31
local.description.notesImported from ARIES
local.identifier.absfor110902 - Cellular Nervous System
local.identifier.ariespublicationu4897219xPUB4
local.identifier.citationvolume588
local.identifier.doi10.1113/jphysiol.2010.188300
local.identifier.scopusID2-s2.0-77953961412
local.identifier.thomsonID000277715100006
local.identifier.uidSubmittedByu4897219
local.type.statusPublished Version

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