Signalling organelle for retrograde axonal transport of internalized neurotrophins from the nerve terminal

dc.contributor.authorSandow, Shaun L
dc.contributor.authorHeydon, Katharina
dc.contributor.authorWeible, Michael
dc.contributor.authorReynolds, Anna
dc.contributor.authorBartlett, Selena
dc.contributor.authorHendry, Ian
dc.date.accessioned2015-12-08T22:33:14Z
dc.date.issued2000
dc.date.updated2015-12-08T09:34:06Z
dc.description.abstractThe retrograde axonal transport of neurotrophins occurs after receptor-mediated endocytosis into vesicles at the nerve terminal. We have been investigating the process of targeting these vesicles for retrograde transport, by examining the transport of [125I]-labelled neurotrophins from the eye to sympathetic and sensory ganglia. With the aid of confocal microscopy, we examined the phenomena further in cultures of dissociated I sympathetic ganglia to which rhodamine-labelled nerve growth factor (NGF) was added. We found the label in large vesicles in the growth cone and axons. Light microscopic examination of the sympathetic nerve trunk in vivo also showed the retrogradely transported material to be sporadically located in large structures in the axons. Ultra-structural examination of the sympathetic nerve trunk after the transport of NGF bound to gold particles showed the label to be concentrated in relatively few large organelles that consisted of accumulations of multivesicular bodies. These results suggest that in vivo NGF is transported in specialized organelles that require assembly in the nerve terminal.
dc.identifier.issn0818-9641
dc.identifier.urihttp://hdl.handle.net/1885/34595
dc.publisherBlackwell Publishing Ltd
dc.sourceImmunology and Cell Biology
dc.subjectKeywords: iodine 125; nerve growth factor; neurotrophin; animal cell; conference paper; confocal microscopy; electron microscopy; mouse; nerve cell; nerve ending; nerve fiber transport; newborn; nonhuman; signal transduction; Animals; Animals, Newborn; Axonal Trans Confocal microscopy; Electron microscopy; Nerve growth factor; Retrograde axonal transport; Vesicle recycling
dc.titleSignalling organelle for retrograde axonal transport of internalized neurotrophins from the nerve terminal
dc.typeJournal article
local.bibliographicCitation.lastpage435
local.bibliographicCitation.startpage430
local.contributor.affiliationSandow, Shaun L, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHeydon, Katharina, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationWeible, Michael, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationReynolds, Anna, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationBartlett, Selena, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHendry, Ian, College of Medicine, Biology and Environment, ANU
local.contributor.authoremailrepository.admin@anu.edu.au
local.contributor.authoruidSandow, Shaun L, u9506167
local.contributor.authoruidHeydon, Katharina, t406
local.contributor.authoruidWeible, Michael, u9910504
local.contributor.authoruidReynolds, Anna, u3050743
local.contributor.authoruidBartlett, Selena, u9505280
local.contributor.authoruidHendry, Ian, u7600265
local.description.embargo2037-12-31
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.absfor060105 - Cell Neurochemistry
local.identifier.ariespublicationMigratedxPub115
local.identifier.citationvolume78
local.identifier.doi10.1046/j.1440-1711.2000.00924.x
local.identifier.scopusID2-s2.0-0033849090
local.identifier.uidSubmittedByMigrated
local.type.statusPublished Version

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