Structure-based rational design of a phosphotriesterase
Date
Authors
Jackson, Colin J
Weir, Khali
Herlt, Anthony
Khurana, Jeevan
Sutherland, Tara D
Horne, Irene
Easton, Christopher
Russell, Robyn
Scott, Colin
Oakeshott, John Graham
Journal Title
Journal ISSN
Volume Title
Publisher
American Society for Microbiology
Abstract
In silico substrate docking of both stereoisomers of the pesticide chlorfenvinphos (CVP) in the phosphotriesterase from Agrobacterium radiobacter identified two residues (F131 and W132) that prevent productive substrate binding and cause stereospecificity. A variant (W131H/F132A) was designed that exhibited ca. 480-fold and 8-fold increases in the rate of Z-CVP and E-CVP hydrolysis, respectively, eliminating stereospecificity.
Description
Citation
Collections
Source
Applied and Environmental Microbiology
Type
Book Title
Entity type
Access Statement
License Rights
Restricted until
2037-12-31
Downloads
File
Description