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Biosynthetically directed 2 H labelling for stereospecific resonance assignments of glycine methylene groups

Date

Authors

Loscha, Karin

Otting, Gottfried

Publisher

Kluwer Academic Publishers

Abstract

Stereospecific resonance assignments of the α-protons of glycine are often difficult to obtain by measurements of scalar coupling constants or nuclear Overhauser effects. Here we show that these stereospecific resonance assignments can readily be obtaine

Keywords

Keywords: carbene; deuterium; glycine; glycine hydroxymethyltransferase; glycine methylene; hydrogen; protein; tetrahydrofolic acid; unclassified drug; article; biosynthesis; carbon nuclear magnetic resonance; controlled study; Escherichia coli; nitrogen nuclear ma Biosynthetically directed 2H labelling; Cell-free protein synthesis; Glycine-2H; NMR spectroscopy; Stereospecific resonance assignments

URI

http://hdl.handle.net/1885/70713

Collections

ANU Research Publications

Source

Journal of Biomolecular NMR

Type

Journal article

DOI

10.1007/s10858-012-9690-x

Restricted until

2037-12-31

Downloads

File

Description

01_Loscha_Biosynthetically_directed__2_H_2013.pdf (267.94 KB)