Inhibition of glutamine transport depletes glutamate and GABA neurotransmitter pools: further evidence for metabolic compartmentation

Date

2003

Authors

Rae, Caroline
Hare, Nathan
Bubb, William A
McEwan, Sally R
Broer, Angelika
McQuillan, James A
Balcar, Vladimir J
Conigrave, Arthur D
Broer, Stefan

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Volume Title

Publisher

Blackwell Publishing Ltd

Abstract

The role of glutamine and alanine transport in the recycling of neurotransmitter glutamate was investigated in Guinea pig brain cortical tissue slices and prisms, and in cultured neuroblastoma and astrocyte cell lines. The ability of exogenous (2 mM) glutamine to displace 13C label supplied as [3-13C] pyruvate, [2-13C]acetate, L-[3-13C] lactate, or D-[1-13C]glucose was investigated using NMR spectroscopy. Glutamine transport was inhibited in slices under quiescent or depolarising conditions using histidine, which shares most transport routes with glutamine, or 2-(methylamino)isobutyric acid (MeAIB), a specific inhibitor of the neuronal system A. Glutamine mainly entered a large, slow turnover pool, probably located in neurons, which did not interact with the glutamate/glutamine neurotransmitter cycle. This uptake was inhibited by MeAIB. When [1-13C]glucose was used as substrate, glutamate/glutamine cycle turnover was inhibited by histidine but not MeAIB, suggesting that neuronal system A may not play a prominent role in neurotransmitter cycling. When transport was blocked by histidine under depolarising conditions, neurotransmitter pools were depleted, showing that glutamine transport is essential for maintenance of glutamate, GABA and alanine pools. Alanine labelling and release were decreased by histidine, showing that alanine was released from neurons and returned to astrocytes. The resultant implications for metabolic compartmentation and regulation of metabolism by transport processes are discussed.

Description

Keywords

Keywords: 2 methyl 2 methylaminopropionic acid; 4 aminobutyric acid; acetic acid; alanine; glucose; glutamic acid; histidine; lactic acid; pyruvic acid; amino acid transport; animal cell; animal tissue; article; astrocyte; brain metabolism; brain slice; controlled Alanine transport; Glutamate/glutamine cycle; Glutamine transport; Metabolic compartmentation; System A

Citation

Source

Journal of Neurochemistry

Type

Journal article

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