Cation-selective ion channels formed by p7 of hepatitis C virus are blocked by hexamethylene amiloride

Date

2004

Authors

Premkumar, Anita
Wilson, L
Ewart, Gary
Gage, Peter

Journal Title

Journal ISSN

Volume Title

Publisher

Elsevier

Abstract

A 63 residue peptide, p7, encoded by hepatitis C virus was synthesised and tested for ion channel activity in lipid bilayer membranes. Ion channels formed by p7 had a variable conductance: some channels had conductances as low as 14 pS. The reversal potential of currents flowing through the channels formed by p7 showed that they were permeable to potassium and sodium ions and less permeable to calcium ions. Addition of Ca2+ to solutions made channels formed by p7 less potassium- or sodium-selective. Hexamethylene amiloride, a drug previously shown to block ion channels formed by Vpu encoded by HIV-1, blocked channels formed by p7. In view of the increasing number of peptides encoded by viruses that have been shown to form ion channels, it is suggested that ion channels may play an important role in the life cycle of many viruses and that drugs that block these channels may prove to be useful antiviral agents.

Description

Keywords

Keywords: amiloride derivative; calcium ion; cation; hexamethylene amiloride; ion channel; potassium ion; protein p7; sodium ion; unclassified drug; virus protein; Vpu protein; article; controlled study; Hepatitis C virus; Human immunodeficiency virus 1; ion conduc Cation-selective ion channels; Hepatitis C virus; Hexamethylene amiloride; p7

Citation

Source

FEBS Letters

Type

Journal article

Book Title

Entity type

Access Statement

License Rights

DOI

10.1016/S0014-5793(03)01453-4

Restricted until

2037-12-31