Spleen stromal cells support haemopoiesis and in vitro growth of dendritic cells from bone marrow

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dc.contributor.authorNi, Keping
dc.contributor.authorO'Neill, Helen
dc.date.accessioned2015-12-13T23:23:59Z
dc.date.issued2000
dc.date.updated2015-12-12T09:18:48Z
dc.description.abstractLong-term stroma-dependent cultures from murine spleen have been previously shown to support dendritic cell (DC) development in vitro. Secondary cultures have now been established using a splenic stromal cell layer overlaid with cells from different lymphoid sites. Cells resembling DCs can be generated in vitro from unfractionated murine lymphoid cells in the absence of added growth factors. Bone marrow (BM) cultures are the most successful but some cultures have been derived from spleen and thymus. Large numbers of mononuclear cells with dendritic morphology can be generated from BM within 2 weeks and cell production in many cultures has been maintained for at least 6 months. A significant proportion of cells binds antibodies specific for DC markers. No lymphoid cells, mast cells or granulocytes are detectable in culture by antibody and histochemical staining and light and electron microscopy. As with cells generated in primary cultures, cells grown in secondary cultures are equally potent stimulators of both syngeneic and allogeneic mixed lymphocyte reactions, confirming their function as antigen- presenting cells. They are also capable of endocytosing and presenting protein antigen to the D10.G4.1 Th2 clone and to unprimed T cells. This study confirmed the presence of DC precursors in multiple lymphoid sites which can be induced to proliferate in the presence of a spleen stromal cell monolayer. The secondary culture system provides an ideal in vitro model for investigation of the development of DC from different tissue sites. It also provides a stable and continuous resource of cells for further studies on DC development.
dc.identifier.issn0007-1048
dc.identifier.urihttp://hdl.handle.net/1885/92025
dc.publisherBlackwell Publishing Ltd
dc.sourceBritish Journal of Haematology
dc.subjectKeywords: animal cell; antigen presentation; article; bone marrow cell; bone marrow culture; cell proliferation; dendritic cell; electron microscopy; endocytosis; hematopoiesis; mixed lymphocyte reaction; mouse; nonhuman; priority journal; spleen; stroma cell; Anim Bone marrow; Dendritic cells; Haemopoiesis; Long-term culture; Stroma
dc.titleSpleen stromal cells support haemopoiesis and in vitro growth of dendritic cells from bone marrow
dc.typeJournal article
local.bibliographicCitation.lastpage67
local.bibliographicCitation.startpage58
local.contributor.affiliationNi, Keping, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationO'Neill, Helen, College of Medicine, Biology and Environment, ANU
local.contributor.authoremailu8100137@anu.edu.au
local.contributor.authoruidNi, Keping, u9105774
local.contributor.authoruidO'Neill, Helen, u8100137
local.description.embargo2037-12-31
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.absfor110202 - Haematology
local.identifier.ariespublicationMigratedxPub22984
local.identifier.citationvolume105
local.identifier.doi10.1111/j.1365-2141.1999.01294.x
local.identifier.scopusID2-s2.0-0032942712
local.identifier.uidSubmittedByMigrated
local.type.statusPublished Version

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