Molecular techniques and their limitations shape our view of the holobiont
Date
2019-07-16
Authors
Cooke, Ira
Mead, Oliver
Whalen, Casey
Boote, Chloe
Moya, A.
Ying, Hua
Robbins, Steven J.
Strugnell, Jan M.
Darling, Aaron
Miller, David J.
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Publisher
Urban & Fischer Verlag
Abstract
It is now recognised that the biology of almost any organism cannot be fully understood without recognising the existence and potential functional importance of associated microbes. Arguably, the emergence of this holistic viewpoint may never have occurred without the development of a crucial molecular technique, 16S rDNA amplicon sequencing, which allowed microbial communities to be easily profiled across a broad range of contexts. A diverse array of molecular techniques are now used to profile microbial communities, infer their evolutionary histories, visualise them in host tissues, and measure their molecular activity. In this review, we examine each of these categories of measurement and inference with a focus on the questions they make tractable, and the degree to which their capabilities and limitations shape our view of the holobiont.
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Keywords
Holobiont, Imaging, Metagenomic sequencing, Evolutionary inference, Model system, Multi-omics
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Source
Zoology
Type
Journal article
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Restricted until
2037-12-31
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