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Fatty acid profiling of Chlamydomonas reinhardtii under nitrogen deprivation

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dc.contributor.authorJames, Gabriel
dc.contributor.authorHocart, Charles
dc.contributor.authorHillier, Warwick
dc.contributor.authorChen, Hancai
dc.contributor.authorKordbacheh, Farzaneh
dc.contributor.authorPrice, Graeme (Dean)
dc.contributor.authorDjordjevic, Michael
dc.date.accessioned2015-12-08T22:08:54Z
dc.date.issued2011
dc.date.updated2016-02-24T11:29:18Z
dc.description.abstractThe Chlamydomonas reinhardtii starch-less mutant, BAF-J5, was found to store lipids up to 65% of dry cell weight when grown photoheterotrophically and subjected to nitrogen starvation. Fourier transform infrared spectroscopy was used as a high-throughput method for semi-quantitative measurements of protein, carbohydrate and lipid content. The fatty acids of wild-type and starch mutants were identified and quantified by gas chromatography mass spectrometry. C. reinhardtii starch mutants, BAF-J5 and I7, produce significantly elevated levels of 16:0, 18:1D9 , 18:2D9,12 and 18:3D9,12,15 fatty acids. Long-chain saturated, mono- and polyunsaturated fatty acids were found under nitrogen starvation. Oleosin-like and caleosin-like genes were identified in the C. reinhardtii genome. However, proteomic analysis of isolated lipid bodies only identified a key lipid droplet associated protein. This study shows it is possible to manipulate algal biosynthetic pathways to produce high levels of lipid that may be suitable for conversion to liquid fuels.
dc.identifier.issn0960-8524
dc.identifier.urihttp://hdl.handle.net/1885/28793
dc.publisherElsevier
dc.sourceBioresource Technology
dc.subjectKeywords: Biosynthetic pathway; Caleosin; Chlamydomonas reinhardtii; Dry cells; Elevated level; Gas chromatography-mass spectrometry; GC/MS; High-throughput method; Lipid bodies; Lipid content; Lipid droplets; Neutral lipid; Nitrogen starvation; Polyunsaturated fat Biofuel; Chlamydomonas reinhardtii; Fatty acid; GC/MS; Neutral lipid
dc.titleFatty acid profiling of Chlamydomonas reinhardtii under nitrogen deprivation
dc.typeJournal article
local.bibliographicCitation.issue3
local.bibliographicCitation.lastpage335
local.bibliographicCitation.startpage3343
local.contributor.affiliationJames, Gabriel, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHocart, Charles, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHillier, Warwick, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationChen, Hancai, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationKordbacheh, Farzaneh, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationPrice, Graeme (Dean), College of Medicine, Biology and Environment, ANU
local.contributor.affiliationDjordjevic, Michael, College of Medicine, Biology and Environment, ANU
local.contributor.authoruidJames, Gabriel, u4490126
local.contributor.authoruidHocart, Charles, u8101127
local.contributor.authoruidHillier, Warwick, u3465503
local.contributor.authoruidChen, Hancai, u8305284
local.contributor.authoruidKordbacheh, Farzaneh, u4650483
local.contributor.authoruidPrice, Graeme (Dean), u8201788
local.contributor.authoruidDjordjevic, Michael, u8400044
local.description.embargo2037-12-31
local.description.notesImported from ARIES
local.identifier.absfor060701 - Phycology (incl. Marine Grasses)
local.identifier.absfor060702 - Plant Cell and Molecular Biology
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
local.identifier.ariespublicationu4956746xPUB60
local.identifier.citationvolume0nline
local.identifier.doi10.1016/j.biortech.2010.11.051
local.identifier.scopusID2-s2.0-78650826459
local.identifier.thomsonID000286904500170
local.type.statusPublished Version

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