Correction and reporting of potassium results in haemolysed samples
dc.contributor.author | Dimeski, Goce | |
dc.contributor.author | Clague, Alan E | |
dc.contributor.author | Hickman, Peter | |
dc.date.accessioned | 2015-12-13T22:59:02Z | |
dc.date.issued | 2005 | |
dc.date.updated | 2015-12-12T07:26:01Z | |
dc.description.abstract | Background: Potassium is usually the most important analyte affected by in vitro haemolysis and the result obtained may falsely indicate or disguise a life-threatening abnormality and so give rise to inappropriate treatment. The purpose of the study was to provide a solution to the problem of reporting potassium on haemolysed samples, taking into account both clinical needs and analytical concerns (inter-individual and inter-sample variability). Methods: Using a new procedure that mimics the collection process in an actual clinical setting, haemolysed samples were prepared from 41 volunteers with a range of inter-individual factors - haemoglobin 80-173g/L, red blood cells 2.42-6.77 × 1012/L, leucocytes 3.0-306 × 109/L and platelets 31-710 × 109/L - in order to develop a more accurate correction equation using a haemolytic index (HI) corresponding to g Hb/L in plasma. Results: The mean (range) potassium increase was 0.0036 mmol/L (0.0029-0.0053 mmol/L) per unit HI. The following equation was developed to estimate potassium increase per HI, in order to compensate approximately for potassium leakage in haemolysed samples: Corrected K+ = Measured K+-(HI × 0.004). Conclusion: The balanced solution is this: instead of reporting the post-haemolysis corrected potassium result a qualitative comment is given, indicating the likely range of the potassium concentration. If the potassium result is in a critically low or high range, it is communicated promptly to the requesting clinician. | |
dc.identifier.issn | 0004-5632 | |
dc.identifier.uri | http://hdl.handle.net/1885/83569 | |
dc.publisher | Royal Society of Medicine Press Ltd | |
dc.source | Annals Of Clinical Biochemistry | |
dc.subject | Keywords: potassium; accuracy; analytic method; article; clinical article; erythrocyte; hemolysis; human; hyperkalemia; laboratory test; observer variation; priority journal; Blood Specimen Collection; False Positive Reactions; Hemolysis; Humans; Hyperkalemia; Math | |
dc.title | Correction and reporting of potassium results in haemolysed samples | |
dc.type | Journal article | |
local.bibliographicCitation.lastpage | 123 | |
local.bibliographicCitation.startpage | 119 | |
local.contributor.affiliation | Dimeski, Goce, Princess Alexandra Hospital | |
local.contributor.affiliation | Clague, Alan E, Princess Alexandra Hospital | |
local.contributor.affiliation | Hickman, Peter, College of Medicine, Biology and Environment, ANU | |
local.contributor.authoremail | repository.admin@anu.edu.au | |
local.contributor.authoruid | Hickman, Peter, a168957 | |
local.description.embargo | 2037-12-31 | |
local.description.notes | Imported from ARIES | |
local.description.refereed | Yes | |
local.identifier.absfor | 110202 - Haematology | |
local.identifier.ariespublication | MigratedxPub11858 | |
local.identifier.citationvolume | 42 | |
local.identifier.doi | 10.1258/0004563053492739 | |
local.identifier.scopusID | 2-s2.0-17144371015 | |
local.identifier.uidSubmittedBy | Migrated | |
local.type.status | Published Version |
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