An environmental DNA assay for detecting Macquarie perch, Macquaria australasica

Date

2017

Authors

Piggott, Maxine

Journal Title

Journal ISSN

Volume Title

Publisher

Springer

Abstract

Macquarie perch (Macquaria australasica) are an endangered freshwater fish found in the Murray-Darling Basin, Australia. I developed a species-specific assay to detect Macquarie perch DNA in environmental samples. The assay is highly specific to detect Macquarie perch, and produced no amplification from 18 co-distributed species. Testing the assay on environmental water samples demonstrated positive detections where Macquarie perch are known to be present in low and moderate density.

Description

Keywords

eDNA, Environmental DNA, Macquarie perch, Macquaria australasica, qPCR

Citation

Source

Conservation Genetics Resources

Type

Journal article

Book Title

Entity type

Access Statement

License Rights

Restricted until

2099-12-31