Tiliqua rugosa microsatellites: isolation via enrichment and characterisation of loci for multiplex PCR in T. rugosa and the endangered T. adelaidensis
Date
2008
Authors
Gardner, Michael
Sanchez, J.J.
Dudaniec, Rachael Y.
Rheinberger, Leah
Smith, Annabel
Saint, Kathleen M.
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Publisher
Kluwer Academic Publishers
Abstract
We used an enrichment technique to isolate 18 novel di and tri microsatellites for the socially monogamous lizard Tiliqua rugosa. These loci were amplified in conjunction with previously described loci in two and three PCR multiplexes for T. rugosa and the endangered T. adelaidensis, respectively. The loci were highly polymorphic in both species, exhibiting between 2 and 32 alleles with observed heterozygosity ranging from 0.43 to 0.96. These markers will be useful for population-level analyses and can contribute to a genetic foundation for conservation strategies for the endangered T. adelaidensis.
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Keywords
Keywords: conservation genetics; endangered species; genetic marker; heterozygosity; lizard; monogamy; polymerase chain reaction; polymorphism; species conservation; Egernia; Squamata; Tiliqua; Tiliqua adelaidensis; Trachydosaurus rugosus Egernia; Microsatellite enrichment; Monogamy; Multiplex PCR; Sociality; Tiliqua
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Source
Conservation Genetics
Type
Journal article
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2037-12-31
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