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Tiliqua rugosa microsatellites: isolation via enrichment and characterisation of loci for multiplex PCR in T. rugosa and the endangered T. adelaidensis

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Date

2008

Authors

Gardner, Michael
Sanchez, J.J.
Dudaniec, Rachael Y.
Rheinberger, Leah
Smith, Annabel
Saint, Kathleen M.

Journal Title

Journal ISSN

Volume Title

Publisher

Kluwer Academic Publishers

Abstract

We used an enrichment technique to isolate 18 novel di and tri microsatellites for the socially monogamous lizard Tiliqua rugosa. These loci were amplified in conjunction with previously described loci in two and three PCR multiplexes for T. rugosa and the endangered T. adelaidensis, respectively. The loci were highly polymorphic in both species, exhibiting between 2 and 32 alleles with observed heterozygosity ranging from 0.43 to 0.96. These markers will be useful for population-level analyses and can contribute to a genetic foundation for conservation strategies for the endangered T. adelaidensis.

Description

Keywords

Keywords: conservation genetics; endangered species; genetic marker; heterozygosity; lizard; monogamy; polymerase chain reaction; polymorphism; species conservation; Egernia; Squamata; Tiliqua; Tiliqua adelaidensis; Trachydosaurus rugosus Egernia; Microsatellite enrichment; Monogamy; Multiplex PCR; Sociality; Tiliqua

Citation

URI

http://hdl.handle.net/1885/65758

Collections

ANU Research Publications

Source

Conservation Genetics

Type

Journal article

Book Title

Entity type

Access Statement

License Rights

DOI

10.1007/s10592-007-9316-0

Restricted until

2037-12-31

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01_Gardner_Tiliqua_rugosa__2008.pdf (53.68 KB)

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