Effect of diet and gut dynamics on the establishment and persistence of Escherichia coli

dc.contributor.authorO'Brien, Claire
dc.contributor.authorGordon, David
dc.date.accessioned2015-12-10T23:07:00Z
dc.date.issued2011
dc.date.updated2016-02-24T12:08:12Z
dc.description.abstractEscherichia coli population dynamics and diversity in rats fed diets differing in their crude fibre content were assessed. Female Wistar rats (n=40) were fed diets containing 1, 4, 18 or 26% crude fibre. Animals were housed in pairs, and one animal was inoculated with a phylogroup B1 strain of E. coli, the other with a phylogroup B2 strain. Natural strain transmission was allowed to occur between the animals in each cage. As expected, the diets had a significant effect on gut dynamics. Mean gut retention times were shorter in animals fed the 18 and 26% crude fibre diets compared with animals on the low-fibre diets. The effect of diet on gastrointestinal dynamics in turn affected E. coli population dynamics and clonal composition. Animals fed the low-fibre diets had higher cell densities than animals fed the high-fibre diets. E. coli populations dominated by phylogroup B2 strains exhibited lower cell densities in animals fed the high-fibre diets compared with cell densities in animals fed the low-fibre diets. Overall, E. coli cell densities declined as gut transit times decreased. Results from this experiment support the results garnered from prospective studies examining the distribution of E. coli from hosts with differing diets, gut morphology and dynamics.
dc.identifier.issn1350-0872
dc.identifier.urihttp://hdl.handle.net/1885/62903
dc.publisherSociety for General Microbiology
dc.sourceMicrobiology (UK)
dc.subjectKeywords: animal experiment; article; bacterial count; bacterial strain; bacterial transmission; cell density; controlled study; dietary fiber; Escherichia coli; female; high fiber diet; inoculation; intestine function; intestine transit time; microbial population
dc.titleEffect of diet and gut dynamics on the establishment and persistence of Escherichia coli
dc.typeJournal article
local.bibliographicCitation.lastpage1384
local.bibliographicCitation.startpage1375
local.contributor.affiliationO'Brien, Claire, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationGordon, David, College of Medicine, Biology and Environment, ANU
local.contributor.authoremailu3244555@anu.edu.au
local.contributor.authoruidO'Brien, Claire, u3244555
local.contributor.authoruidGordon, David, u9308141
local.description.embargo2037-12-31
local.description.notesImported from ARIES
local.identifier.absfor060504 - Microbial Ecology
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
local.identifier.ariespublicationu9511635xPUB748
local.identifier.citationvolume157
local.identifier.doi10.1099/mic.0.047092-0
local.identifier.scopusID2-s2.0-79955756344
local.identifier.thomsonID000291179900013
local.identifier.uidSubmittedByu9511635
local.type.statusPublished Version

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