Genomics-Driven Discovery of Phytotoxic Cytochalasans Involved in the Virulence of the Wheat Pathogen Parastagonospora nodorum
dc.contributor.author | Li, Hang | |
dc.contributor.author | Wei, Haochen | |
dc.contributor.author | Hu, Jinyu | |
dc.contributor.author | Lacey, E | |
dc.contributor.author | Sobolev, Alexandre N | |
dc.contributor.author | Stubbs, Keith A | |
dc.contributor.author | Solomon, Peter | |
dc.contributor.author | Chooi, Yit-Heng | |
dc.date.accessioned | 2020-08-21T00:00:34Z | |
dc.date.issued | 2020 | |
dc.date.updated | 2020-04-28T17:48:09Z | |
dc.description.abstract | The etiology of fungal pathogenesis of grains is critical to global food security. The large number of orphan biosynthetic gene clusters uncovered in fungal plant pathogen genome sequencing projects suggests that we have a significant knowledge gap about the secondary metabolite repertoires of these pathogens and their roles in plant pathogenesis. Cytochalasans are a family of natural products of significant interest due to their ability to bind to actin and interfere with cellular processes that involved actin polymerization; however, our understanding of their biosynthesis and biological roles remains incomplete. Here, we identified a putative polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) gene cluster (phm) that was upregulated in the pathogen Parastagonospora nodorum during its infection on wheat. Overexpression of the transcription factor gene phmR encoded in the phm gene cluster resulted in the production of two leucine-derived cytochalasans, phomacins D and E (1 and 2, respectively), and an acetonyl adduct phomacin F. Heterologous expression of the PKS-NRPS gene phmA and the trans-enoyl reductase (ER) gene phmE in Aspergillus nidulans resulted in the production of a novel 2-pyrrolidone precursor prephomacin. Reverse genetics and wheat seedling infection assays showed that ΔphmA mutants exhibited significantly reduced virulence compared to the wild type. We further demonstrated that both 1 and 2 showed potent actin polymerization-inhibitory activities and exhibited potentially monocot-specific antigerminative activities. The findings from this study have advanced our knowledge based on the biosynthesis and biological roles of cytochalasans, the latter of which could have significant implications for our understanding of the molecular mechanisms of fungus−plant interactions. | en_AU |
dc.description.sponsorship | This study was funded by an Australian Research Council (ARC) Discovery Project Grant (DP170100228). Y.-H.C. is an ARC Future Fellow (FT160100233). H.L. and J.H. are supported by an Australian Government International Postgraduate Research Scholarship. | en_AU |
dc.format.mimetype | application/pdf | en_AU |
dc.identifier.issn | 1554-8929 | en_AU |
dc.identifier.uri | http://hdl.handle.net/1885/207907 | |
dc.language.iso | en_AU | en_AU |
dc.publisher | American Chemical Society | en_AU |
dc.relation | http://purl.org/au-research/grants/arc/DP170100228 | en_AU |
dc.relation | http://purl.org/au-research/grants/arc/FT160100233 | en_AU |
dc.rights | © 2019 American Chemical Society | en_AU |
dc.source | ACS chemical biology | en_AU |
dc.title | Genomics-Driven Discovery of Phytotoxic Cytochalasans Involved in the Virulence of the Wheat Pathogen Parastagonospora nodorum | en_AU |
dc.type | Journal article | en_AU |
local.bibliographicCitation.lastpage | 233 | en_AU |
local.bibliographicCitation.startpage | 226 | en_AU |
local.contributor.affiliation | Li, Hang, The University of Western Australia | en_AU |
local.contributor.affiliation | Wei, Haochen, College of Science, ANU | en_AU |
local.contributor.affiliation | Hu, Jinyu, University of Western Australia, | en_AU |
local.contributor.affiliation | Lacey, E, Microbial Screening Technol. Pty Ltd. | en_AU |
local.contributor.affiliation | Sobolev, Alexandre N, University of Western Australia | en_AU |
local.contributor.affiliation | Stubbs, Keith A, University of Western Australia | en_AU |
local.contributor.affiliation | Solomon, Peter, College of Science, ANU | en_AU |
local.contributor.affiliation | Chooi, Yit-Heng, The University of Western Australia | en_AU |
local.contributor.authoremail | u4632004@anu.edu.au | en_AU |
local.contributor.authoruid | Wei, Haochen, u6030062 | en_AU |
local.contributor.authoruid | Solomon, Peter, u4632004 | en_AU |
local.description.embargo | 2037-12-31 | |
local.description.notes | Imported from ARIES | en_AU |
local.identifier.absfor | 060113 - Synthetic Biology | en_AU |
local.identifier.absfor | 060704 - Plant Pathology | en_AU |
local.identifier.absseo | 820599 - Winter Grains and Oilseeds not elsewhere classified | en_AU |
local.identifier.ariespublication | u6269649xPUB392 | en_AU |
local.identifier.citationvolume | 15 | en_AU |
local.identifier.doi | 10.1021/acschembio.9b00791 | en_AU |
local.identifier.scopusID | 2-s2.0-85077221416 | |
local.identifier.uidSubmittedBy | u6269649 | en_AU |
local.publisher.url | http://pubs.acs.org/journal/acbcct | en_AU |
local.type.status | Published Version | en_AU |
Downloads
Original bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- 01_Li_Genomics-Driven_Discovery_of_2020.pdf
- Size:
- 3.66 MB
- Format:
- Adobe Portable Document Format