Isovitexin-2'- O -β-[6- O -E- p - coumaroylglucopyranoside] from UV-B irradiated Leaves of Rice, Oryza sativa L. inhibits Fertility of Helicoverpa armigera

Abstract

UV-B irradiated rice leaves (Oryza sativa L.) contained four closely related flavonoids, with either an isoorientin or isovitexin aglycone. These flavonoids have previously been purified and characterized, and were added to artificial diets of the African bollworm (Helicoverpa armigera Hübner) at 0.1x concentration found in irradiated rice leaves. Consumption of different diets had relatively small effects on laval, pupal and adult duration, weight and survival, indicating the insects lived near normal life cycles on all diets. However, one of the compounds, flavonoid IIa, isovitexin-2′-O-β-[6- O-E-p-coumaroylglucopyranoside], dramatically reduced the number of fertile eggs laid to 7% of control insects (P < 0.001) when added to insect diets at 18 nmol gFW-1 (14 ppm). A similar antifertility effect was observed when only the male partner consumed diet containing flavonoid IIa, indicating that the reduced fertility may be male specific. In contrast, the fecundity and fertility of insects eating diets containing the closely related flavonoids, isoorientin-2′-O-β-[6-O-E-p-coumaroylglucopyranoside] or isoorientin-2′-O-β-[6-O-E-p-feruloylglucopyranoside], were not significantly different to control diets.