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Optical trapping and surgery of living yeast cells using a single laser

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dc.contributor.authorAndo, Jun
dc.contributor.authorBautista, Godofredo
dc.contributor.authorSmith, Nicholas
dc.contributor.authorFujita, Katsumasa
dc.contributor.authorDaria, Vincent Ricardo
dc.date.accessioned2015-12-04T03:50:53Z
dc.date.available2015-12-04T03:50:53Z
dc.date.issued2008-10-20
dc.date.updated2015-12-08T02:46:21Z
dc.description.abstractWe present optical trapping and surgery of living yeast cells using two operational modes of a single laser. We used a focused laser beam operating in continuous-wave mode for noninvasive optical trapping and manipulation of single yeast cell. We verified that such operational mode of the laser does not cause any destructive effect on yeast cell wall. By changing the operation of the laser to femtosecond-pulsed mode, we show that a tightly focused beam dissects the yeast cell walls via nonlinear absorption. Lastly, using the combined technique of optical microsurgery and trapping, we demonstrate intracellular organelle extraction and manipulation from a yeast cell. The technique established here will be useful as an efficient method for both surgery and manipulation of living cells using a single laser beam.
dc.description.sponsorshipThe project has been funded by the Philippine Council for Advanced Science and Technology Research and Development PCASTRD. J. Ando acknowledges the support of the Japan Student Services Organization JASSO for the short-term student exchange promotion program.en_AU
dc.identifier.issn0034-6748en_AU
dc.identifier.urihttp://hdl.handle.net/1885/17012
dc.publisherAmerican Institute of Physics (AIP)
dc.rightshttp://www.sherpa.ac.uk/romeo/issn/0034-6748..."Publishers version/PDF may be used on author's personal website, institutional website or institutional repository" from SHERPA/RoMEO site (as at 4/12/15). Copyright 2008 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Review of Scientific Instruments and may be found at https://doi.org/10.1063/1.2999542
dc.sourceReview of Scientific Instruments
dc.subjectcell wall
dc.subjectcytological techniques
dc.subjectmicroscopy, video
dc.subjectoptics and photonics
dc.subjectorganelles
dc.subjectsaccharomyces cerevisiae
dc.subjectlasers
dc.titleOptical trapping and surgery of living yeast cells using a single laser
dc.typeJournal article
local.bibliographicCitation.issue10en_AU
local.bibliographicCitation.lastpage103711
local.bibliographicCitation.startpage103705en_AU
local.contributor.affiliationAndo, Jun, Osaka University, Japanen_AU
local.contributor.affiliationBautista, Godofredo, University of the Philippines, Philippinesen_AU
local.contributor.affiliationSmith, Nicholas, Osaka University, Japanen_AU
local.contributor.affiliationFujita, Katsumasa, Osaka University, Japanen_AU
local.contributor.affiliationDaria, Vincent, College of Medicine, Biology and Environment, CMBE John Curtin School of Medical Research, Eccles Institute of Neuroscience, The Australian National Universityen_AU
local.contributor.authoruidu4492652
local.description.notesImported from ARIESen_AU
local.identifier.absfor020502en_AU
local.identifier.ariespublicationu4492652xPUB3en_AU
local.identifier.citationvolume79en_AU
local.identifier.doi10.1063/1.2999542en_AU
local.identifier.essn1089-7623en_AU
local.identifier.scopusID2-s2.0-55349099163
local.identifier.thomsonID000260573500023
local.publisher.urlhttps://www.aip.org/en_AU
local.type.statusPublished Versionen_AU

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