Characterization of the chloroquine resistance transporter homologue in Toxoplasma gondii

dc.contributor.authorWarring, Sally D
dc.contributor.authorDou, Zhicheng
dc.contributor.authorCarruthers, Vern B.
dc.contributor.authorMcFadden, Geoffrey I
dc.contributor.authorvan Dooren, Giel
dc.date.accessioned2015-12-13T22:32:44Z
dc.date.issued2014
dc.date.updated2015-12-11T09:09:52Z
dc.description.abstractMutations in the Plasmodium falciparum chloroquine resistance transporter (PfCRT) protein confer resistance to the antima-larial drug chloroquine. PfCRT localizes to the parasite digestive vacuole, the site of chloroquine action, where it mediates resistance by transporting chloroquine out of the digestive vacuole. PfCRT belongs to a family of transporter proteins called the chlo-roquine resistance transporter family. CRT family proteins are found throughout the Apicomplexa, in some protists, and in plants. Despite the importance of PfCRT in drug resistance, little is known about the evolution or native function of CRT proteins. The apicomplexan parasite Toxoplasma gondii contains one CRT family protein. We demonstrate that T. gondii CRT (TgCRT) colocalizes with markers for the vacuolar (VAC) compartment in these parasites. The TgCRT-containing VAC is a highly dynamic organelle, changing its morphology and protein composition between intracellular and extracellular forms of the parasite. Regulated knockdown of TgCRT expression resulted in modest reduction in parasite fitness and swelling of the VAC, indicating that TgCRT contributes to parasite growth and VAC physiology. Together, our findings provide new information on the role of CRT family proteins in apicomplexan parasites.
dc.identifier.issn1535-9778
dc.identifier.urihttp://hdl.handle.net/1885/75709
dc.publisherAmerican Society for Microbiology
dc.rightsAuthor/s retain copyrighten_AU
dc.sourceEukaryotic Cell
dc.titleCharacterization of the chloroquine resistance transporter homologue in Toxoplasma gondii
dc.typeJournal article
dcterms.accessRightsOpen Accessen_AU
local.bibliographicCitation.issue11
local.bibliographicCitation.lastpage1370
local.bibliographicCitation.startpage1360
local.contributor.affiliationWarring, Sally D, School of Botany, University of Melbourne
local.contributor.affiliationDou, Zhicheng, University of Michigan Medical School
local.contributor.affiliationCarruthers, Vern B., University of Michigan School of Medicine
local.contributor.affiliationMcFadden, Geoffrey I, University of Melbourne
local.contributor.affiliationvan Dooren, Giel, College of Medicine, Biology and Environment, ANU
local.contributor.authoremailu5083783@anu.edu.au
local.contributor.authoruidvan Dooren, Giel, u5083783
local.description.notesImported from ARIES
local.identifier.absfor060000 - BIOLOGICAL SCIENCES
local.identifier.ariespublicationU3488905xPUB4757
local.identifier.citationvolume13
local.identifier.doi10.1128/EC.00027-14
local.identifier.scopusID2-s2.0-84907990760
local.identifier.thomsonID000344646600002
local.identifier.uidSubmittedByU3488905
local.type.statusPublished Version

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