Characterization of the chloroquine resistance transporter homologue in Toxoplasma gondii
dc.contributor.author | Warring, Sally D | |
dc.contributor.author | Dou, Zhicheng | |
dc.contributor.author | Carruthers, Vern B. | |
dc.contributor.author | McFadden, Geoffrey I | |
dc.contributor.author | van Dooren, Giel | |
dc.date.accessioned | 2015-12-13T22:32:44Z | |
dc.date.issued | 2014 | |
dc.date.updated | 2015-12-11T09:09:52Z | |
dc.description.abstract | Mutations in the Plasmodium falciparum chloroquine resistance transporter (PfCRT) protein confer resistance to the antima-larial drug chloroquine. PfCRT localizes to the parasite digestive vacuole, the site of chloroquine action, where it mediates resistance by transporting chloroquine out of the digestive vacuole. PfCRT belongs to a family of transporter proteins called the chlo-roquine resistance transporter family. CRT family proteins are found throughout the Apicomplexa, in some protists, and in plants. Despite the importance of PfCRT in drug resistance, little is known about the evolution or native function of CRT proteins. The apicomplexan parasite Toxoplasma gondii contains one CRT family protein. We demonstrate that T. gondii CRT (TgCRT) colocalizes with markers for the vacuolar (VAC) compartment in these parasites. The TgCRT-containing VAC is a highly dynamic organelle, changing its morphology and protein composition between intracellular and extracellular forms of the parasite. Regulated knockdown of TgCRT expression resulted in modest reduction in parasite fitness and swelling of the VAC, indicating that TgCRT contributes to parasite growth and VAC physiology. Together, our findings provide new information on the role of CRT family proteins in apicomplexan parasites. | |
dc.identifier.issn | 1535-9778 | |
dc.identifier.uri | http://hdl.handle.net/1885/75709 | |
dc.publisher | American Society for Microbiology | |
dc.rights | Author/s retain copyright | en_AU |
dc.source | Eukaryotic Cell | |
dc.title | Characterization of the chloroquine resistance transporter homologue in Toxoplasma gondii | |
dc.type | Journal article | |
dcterms.accessRights | Open Access | en_AU |
local.bibliographicCitation.issue | 11 | |
local.bibliographicCitation.lastpage | 1370 | |
local.bibliographicCitation.startpage | 1360 | |
local.contributor.affiliation | Warring, Sally D, School of Botany, University of Melbourne | |
local.contributor.affiliation | Dou, Zhicheng, University of Michigan Medical School | |
local.contributor.affiliation | Carruthers, Vern B., University of Michigan School of Medicine | |
local.contributor.affiliation | McFadden, Geoffrey I, University of Melbourne | |
local.contributor.affiliation | van Dooren, Giel, College of Medicine, Biology and Environment, ANU | |
local.contributor.authoremail | u5083783@anu.edu.au | |
local.contributor.authoruid | van Dooren, Giel, u5083783 | |
local.description.notes | Imported from ARIES | |
local.identifier.absfor | 060000 - BIOLOGICAL SCIENCES | |
local.identifier.ariespublication | U3488905xPUB4757 | |
local.identifier.citationvolume | 13 | |
local.identifier.doi | 10.1128/EC.00027-14 | |
local.identifier.scopusID | 2-s2.0-84907990760 | |
local.identifier.thomsonID | 000344646600002 | |
local.identifier.uidSubmittedBy | U3488905 | |
local.type.status | Published Version |
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