Accumulation of and Response to Auxins in Roots and Nodules of the Actinorhizal Plant Datisca glomerata Compared to the Model Legume Medicago truncatula

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dc.contributor.authorDemina, Irina V.
dc.contributor.authorMaity, Pooja Jha
dc.contributor.authorNagchowdhury, Anurupa
dc.contributor.authorNg, Liang
dc.contributor.authorvan der Graaff, Eric
dc.contributor.authorDemchenko, Kirill N.
dc.contributor.authorRoitsch, Thomas
dc.contributor.authorMathesius, Ulrike
dc.contributor.authorPawlowski, Katharina
dc.date.accessioned2020-05-11T00:38:00Z
dc.date.available2020-05-11T00:38:00Z
dc.date.issued2019-09-24
dc.date.updated2019-12-19T07:45:19Z
dc.description.abstractActinorhizal nodules are structurally different from legume nodules and show a greater similarity to lateral roots. Because of the important role of auxins in lateral root and nodule formation, auxin profiles were examined in roots and nodules of the actinorhizal species Datisca glomerata and the model legume Medicago truncatula. The auxin response in roots and nodules of both species was analyzed in transgenic root systems expressing a beta-glucuronidase gene under control of the synthetic auxin-responsive promoter DR5. The effects of two different auxin on root development were compared for both species. The auxin present in nodules at the highest levels was phenylacetic acid (PAA). No differences were found between the concentrations of active auxins of roots vs. nodules, while levels of the auxin conjugate indole-3-acetic acid-alanine were increased in nodules compared to roots of both species. Because auxins typically act in concert with cytokinins, cytokinins were also quantified. Concentrations of cis-zeatin and some glycosylated cytokinins were dramatically increased in nodules compared to roots of D. glomerata, but not of M. truncatula. The ratio of active auxins to cytokinins remained similar in nodules compared to roots in both species. The auxin response, as shown by the activation of the DR5 promoter, seemed significantly reduced in nodules compared to roots of both species, suggesting the accumulation of auxins in cell types that do not express the signal transduction pathway leading to DR5 activation. Effects on root development were analyzed for the synthetic auxin naphthaleneacetic acid (NAA) and PAA, the dominant auxin in nodules. Both auxins had similar effects, except that the sensitivity of roots to PAA was lower than to NAA. However, while the effects of both auxins on primary root growth were similar for both species, effects on root branching were different: both auxins had the classical positive effect on root branching in M. truncatula, but a negative effect in D. glomerata. Such a negative effect of exogenous auxin on root branching has previously been found for a cucurbit that forms lateral root primordia in the meristem of the parental root; however, root branching in D. glomerata does not follow that pattern.en_AU
dc.description.sponsorshipThis study was supported by two grants from the Swedish Research Council Vetenskapsrådet (VR 2007-17840-52674-16 and VR 2012-03061) and by a grant from Carl Tryggers Stiftelse för Vetenskaplig Forskning (CTS 13:354) to KP, by a grant from the Russian Science Foundation (analyses of auxin response pattern, grant no. 16-16-00089) to KND, and by a grant from the Ministry of Education, Youth and Sports of CR within the National Sustainability Program I (NPUI, grant number LO1415) to TR. UM was supported by the Australian Research Council (DP150102002).en_AU
dc.format.mimetypeapplication/pdfen_AU
dc.identifier.issn1664-462Xen_AU
dc.identifier.urihttp://hdl.handle.net/1885/203962
dc.language.isoen_AUen_AU
dc.provenanceThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_AU
dc.publisherFrontiers Research Foundationen_AU
dc.relationhttp://purl.org/au-research/grants/arc/DP150102002en_AU
dc.rights© 2019 Demina, Maity, Nagchowdhury, Ng, van der Graaff, Demchenko, Roitsch, Mathesius and Pawlowskien_AU
dc.rights.licenseCreative Commons Attribution License (CC BY)en_AU
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_AU
dc.sourceFrontiers in Plant Scienceen_AU
dc.subjectIAAen_AU
dc.subjectPAAen_AU
dc.subjectcytokininsen_AU
dc.subjectDR5:GUSen_AU
dc.subjecthairy rootsen_AU
dc.subjectroot branchingen_AU
dc.subjectnodulesen_AU
dc.titleAccumulation of and Response to Auxins in Roots and Nodules of the Actinorhizal Plant Datisca glomerata Compared to the Model Legume Medicago truncatulaen_AU
dc.typeJournal articleen_AU
dcterms.accessRightsOpen Accessen_AU
dcterms.dateAccepted2019-08-09
local.bibliographicCitation.issue1085en_AU
local.bibliographicCitation.lastpage19en_AU
local.bibliographicCitation.startpage1en_AU
local.contributor.affiliationDemina, Irina V., Stockholm Universityen_AU
local.contributor.affiliationMaity, Pooja Jha, Stockholm Universityen_AU
local.contributor.affiliationNagchowdhury, Anurupa, Stockholm Universityen_AU
local.contributor.affiliationNg, Liang, College of Science, ANUen_AU
local.contributor.affiliationvan der Graaff, Eric, Karl-Franzens-Universitäten_AU
local.contributor.affiliationDemchenko, Kirill N., Russian Academy of Sciencesen_AU
local.contributor.affiliationRoitsch, Thomas, Karl-Franzens-Universitäten_AU
local.contributor.affiliationMathesius, Ulrike, College of Science, ANUen_AU
local.contributor.affiliationPawlowski, Katharina, Stockholm Universityen_AU
local.contributor.authoremailu4348304@anu.edu.auen_AU
local.contributor.authoruidNg, Liang, u4348304en_AU
local.contributor.authoruidMathesius, Ulrike, u9601788en_AU
local.description.notesImported from ARIESen_AU
local.identifier.absfor060702 - Plant Cell and Molecular Biologyen_AU
local.identifier.absfor060703 - Plant Developmental and Reproductive Biologyen_AU
local.identifier.absseo820503 - Grain Legumesen_AU
local.identifier.ariespublicationu5786633xPUB1106en_AU
local.identifier.citationvolume10en_AU
local.identifier.doi10.3389/fpls.2019.01085en_AU
local.identifier.scopusID2-s2.0-85073252550
local.identifier.uidSubmittedByu5786633en_AU
local.publisher.urlhttps://www.frontiersin.org/en_AU
local.type.statusPublished Versionen_AU

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