Cultural advice

The Australian National University acknowledges, celebrates and pays our respects to the Ngunnawal and Ngambri people of the Canberra region and to all First Nations Australians on whose traditional lands we meet and work, and whose cultures are among the oldest continuing cultures in human history.

Aboriginal and Torres Strait Islander peoples are advised that ANU Library collections may include images, names, voices, and other representations of deceased persons.

Material in the collection may contain terms, language or views that reflect the period in which the item was created and may be considered inappropriate today.

Structural insights into Escherichia coli phosphopantothenoylcysteine synthetase by native ion mobility-mass spectrometry

Simple item page
dc.contributor.authorChan, Daniel Shiu-Hin
dc.contributor.authorHess, Jeannine
dc.contributor.authorShaw, Elen
dc.contributor.authorSpry, Christina
dc.contributor.authorStarley, Robert
dc.contributor.authorDagostin, Claudio
dc.contributor.authorDias, Marcio V.B.
dc.contributor.authorKale, Ramesh
dc.contributor.authorMendes, Vitor
dc.contributor.authorBlundell, Tom
dc.contributor.authorCoyne, Anthony G.
dc.contributor.authorAbell, Christopher
dc.date.accessioned2021-04-21T04:57:15Z
dc.date.issued2019
dc.date.updated2022-07-24T08:22:02Z
dc.description.abstractCoaBC, part of the vital coenzyme A biosynthetic pathway in bacteria, has recently been validated as a promising antimicrobial target. In this work, we employed native ion mobility–mass spectrometry to gain structural insights into the phosphopantothenoylcysteine synthetase domain of E. coli CoaBC. Moreover, native mass spectrometry was validated as a screening tool to identify novel inhibitors of this enzyme, highlighting the utility and versatility of this technique both for structural biology and for drug discovery.
dc.description.sponsorshipD.S.H.C. acknowledges the support of the Croucher Foundation and the Cambridge Commonwealth, European and International Trust for receipt of a Croucher Cambridge International Scholarship. J.H. was financially supported by the Swiss National Science Foundation (SNSF Early PostDoc. Mobility fellowship, P2ZHP2_164947) and the EU H2020 Framework Programme (H2020-MSCA-IF-2017, ID: 789607) M.V.B.D., C.D., C.S., R.K. and V.M. would like to thank the Bill and Melinda Gates Foundation for funding (SHORTEN-TB OPP1158806). C.S. would also like to thank the National Health and Medical Research Council (Australia) for a NHMRC Overseas Biomedical Fellowship (1016357).en_AU
dc.format.mimetypeapplication/pdfen_AU
dc.identifier.issn0264-6021en_AU
dc.identifier.urihttp://hdl.handle.net/1885/230437
dc.language.isoen_AUen_AU
dc.publisherPortland Press
dc.relationhttp://purl.org/au-research/grants/nhmrc/1016357
dc.rights© 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
dc.sourceBiochemical Journal
dc.titleStructural insights into Escherichia coli phosphopantothenoylcysteine synthetase by native ion mobility-mass spectrometry
dc.typeJournal article
local.bibliographicCitation.issue0264-6021en_AU
local.bibliographicCitation.lastpage3139en_AU
local.bibliographicCitation.startpage3125en_AU
local.contributor.affiliationChan, Daniel Shiu-Hin, University of Cambridgeen_AU
local.contributor.affiliationHess, Jeannine, University of Cambridgeen_AU
local.contributor.affiliationShaw, Elen, University of Cambridgeen_AU
local.contributor.affiliationSpry, Christina, College of Science, ANUen_AU
local.contributor.affiliationStarley, Robert, University of Cambridgeen_AU
local.contributor.affiliationDagostin, Claudio, University of Cambridgeen_AU
local.contributor.affiliationDias, Marcio V.B., University of Cambridgeen_AU
local.contributor.affiliationKale, Ramesh, University of Cambridgeen_AU
local.contributor.affiliationMendes, Vitor, University of Cambridgeen_AU
local.contributor.affiliationBlundell, Tom, University of Cambridgeen_AU
local.contributor.affiliationCoyne, Anthony G., University of Cambridgeen_AU
local.contributor.affiliationAbell, Christopher, University of Cambridgeen_AU
local.contributor.authoruidSpry, Christina, u3359155en_AU
local.description.embargo2099-12-31
local.description.notesImported from ARIESen_AU
local.identifier.absfor111501 - Basic Pharmacologyen_AU
local.identifier.absfor110803 - Medical Parasitologyen_AU
local.identifier.absfor060502 - Infectious Agentsen_AU
local.identifier.absseo920109 - Infectious Diseasesen_AU
local.identifier.ariespublicationu6269649xPUB184en_AU
local.identifier.citationvolume476en_AU
local.identifier.doi10.1042/BCJ20190318en_AU
local.identifier.scopusID2-s2.0-85074621192
local.identifier.thomsonIDWOS:000509877500002
local.publisher.urlhttp://www.portlandpress.com/biochemjen_AU
local.type.statusPublished Versionen_AU

Downloads

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
01_Chan_Structural_insights_into_2019.pdf
Size:
2.42 MB
Format:
Adobe Portable Document Format
Download

Collections

ANU Research Publications

DSpace software copyright © 2002-2026 LYRASIS