IDENTIFICATION OF NOVEL GLUTATHIONE TRANSFERASES AND POLYMORPHIC VARIANTS BY EXPRESSED SEQUENCE TAG DATABASE ANALYSIS

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dc.contributor.authorBoard, Philip
dc.contributor.authorChelvanayagam, Gareth
dc.contributor.authorJermiin, Lars
dc.contributor.authorTetlow, Natasha
dc.contributor.authorTzeng, Huey-Fen
dc.contributor.authorAnders, Michael
dc.contributor.authorBlackburn, Anneke
dc.date.accessioned2015-12-13T23:27:10Z
dc.date.available2015-12-13T23:27:10Z
dc.date.issued2001
dc.date.updated2015-12-12T09:49:25Z
dc.description.abstractThe human expressed sequence tag (EST) database can be searched by different sequence alignment strategies to identify new members of gene families and allelic variants. To illustrate the value of database analysis for gene discovery, we have focused on the glutathione S-transferase (GST) super family, an approach that has led to the identification of the Zeta class. The Zeta class GSTs catalyze the glutathione-dependent biotransformation of α-haloacids and the isomerization of maleylacetoacetic acid to fumarylacetoacetic acid, an essential step in the catabolism of tyrosine. Allelic variants of the GST Z1 and GST A2 genes have also been identified by EST database analysis. One GST Z1 variant (GST Z1A) has significantly higher activity with dichloroacetic acid as a substrate than other GST Z1 isoforms. This variant may be important in the clinical treatment of lactic acidosis where dichloroacetic acid is prescribed. Our experience with the application of EST database searching methods suggests that it may be productively applied to other gene families of pharmacogenetic interest.
dc.identifier.issn0090-9556
dc.identifier.urihttp://hdl.handle.net/1885/93199
dc.publisherAmerican Society for Pharmacology and Experimental Therapeutics
dc.sourceDrug Metabolism and Disposition
dc.subjectKeywords: acetoacetic acid; dichloroacetic acid; fumarulacetoacetic acid; glutathione transferase; haloacid; maleylacetoacetic acid; tyrosine; unclassified drug; allele; biotransformation; catabolism; conference paper; data base; DNA sequence; expressed sequence ta
dc.titleIDENTIFICATION OF NOVEL GLUTATHIONE TRANSFERASES AND POLYMORPHIC VARIANTS BY EXPRESSED SEQUENCE TAG DATABASE ANALYSIS
dc.typeJournal article
local.bibliographicCitation.issue4
local.bibliographicCitation.lastpage547
local.bibliographicCitation.startpage544
local.contributor.affiliationBoard, Philip, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationChelvanayagam, Gareth, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationJermiin, Lars, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationTetlow, Natasha, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationTzeng, Huey-Fen, National Chi Nan University
local.contributor.affiliationAnders, Michael, University of Rochester
local.contributor.affiliationBlackburn, Anneke, College of Medicine, Biology and Environment, ANU
local.contributor.authoremailu7701651@anu.edu.au
local.contributor.authoruidBoard, Philip, u7701651
local.contributor.authoruidChelvanayagam, Gareth, u950151
local.contributor.authoruidJermiin, Lars, u951517
local.contributor.authoruidTetlow, Natasha, u9718329
local.contributor.authoruidBlackburn, Anneke, u4048450
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.absfor060107 - Enzymes
local.identifier.ariespublicationMigratedxPub26563
local.identifier.citationvolume29
local.identifier.scopusID2-s2.0-0035059667
local.identifier.uidSubmittedByMigrated
local.type.statusPublished Version

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