The Transcriptome and Terpene Profile of Eucalyptus grandis Reveals Mechanisms of Defense Against the Insect Pest, Leptocybe invasa
dc.contributor.author | Oates, Caryn N. | |
dc.contributor.author | Kulheim, Carsten | |
dc.contributor.author | Myburg, Alexander A. | |
dc.contributor.author | Slippers, Bernard | |
dc.contributor.author | Naidoo, Sanushka | |
dc.date.accessioned | 2015-12-10T23:25:02Z | |
dc.date.issued | 2015 | |
dc.date.updated | 2015-12-10T10:52:25Z | |
dc.description.abstract | Plants have evolved complex defenses that allow them to protect themselves against pests and pathogens. However, there is relatively little information regarding the Eucalyptus defensome. Leptocybe invasa is one of the most damaging pests in global Eucalyptus forestry, and essentially nothing is known regarding the molecular mechanisms governing the interaction between the pest and host. The aim of the study was to investigate changes in the transcriptional landscape and terpene profile of a resistant and susceptible Eucalyptus genotype in an effort to improve our understanding of this interaction. We used RNA-seqencing to investigate tran-scriptional changes following L. invasa oviposition. Expression levels were validated using real-time quantitative PCR. Terpene profiles were investigated using gas chroma-tography coupled to mass spectometry on uninfested and oviposited leaves. We found 698 and 1,115 significantly differentially expressed genes from the resistant and susceptible interactions, respectively. Gene Ontology enrichment and Mapman analyses identified putative defense mechanisms including cell wall reinforcement, protease inhibitors, cell cycle suppression and regulatory hormone signaling pathways. There were significant differences in the mono- and sesquiterpene profiles between genotypes and between control and infested material. A model of the interaction between Eucalyptus and L. invasa was proposed from the transcriptomic and chemical data. | |
dc.identifier.issn | 0032-0781 | |
dc.identifier.uri | http://hdl.handle.net/1885/67455 | |
dc.publisher | Japanese Society of Plant Physiologists | |
dc.source | Plant and Cell Physiology | |
dc.title | The Transcriptome and Terpene Profile of Eucalyptus grandis Reveals Mechanisms of Defense Against the Insect Pest, Leptocybe invasa | |
dc.type | Journal article | |
local.bibliographicCitation.lastpage | 11 | |
local.bibliographicCitation.startpage | 1 | |
local.contributor.affiliation | Oates, Caryn N., University of Pretoria | |
local.contributor.affiliation | Kulheim, Carsten, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Myburg, Alexander A., University of Pretoria | |
local.contributor.affiliation | Slippers, Bernard, University of Pretoria | |
local.contributor.affiliation | Naidoo, Sanushka, University of Pretoria | |
local.contributor.authoremail | u4569873@anu.edu.au | |
local.contributor.authoruid | Kulheim, Carsten, u4569873 | |
local.description.embargo | 2037-12-31 | |
local.description.notes | Imported from ARIES | |
local.identifier.absfor | 060405 - Gene Expression (incl. Microarray and other genome-wide approaches) | |
local.identifier.absfor | 060702 - Plant Cell and Molecular Biology | |
local.identifier.absfor | 060202 - Community Ecology | |
local.identifier.absseo | 820101 - Hardwood Plantations | |
local.identifier.ariespublication | u9511635xPUB1458 | |
local.identifier.citationvolume | Published online: 5 May 2015 | |
local.identifier.doi | 10.1093/pcp/pcv064 | |
local.identifier.scopusID | 2-s2.0-84942163791 | |
local.identifier.uidSubmittedBy | u9511635 | |
local.type.status | Published Version |