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A quantitative assessment of the carbonic anhydrase activity in photosystem II

Date

2007

Authors

McConnell, Iain
Badger, Murray
Wydrzynski, Thomas
Hillier, Warwick

Journal Title

Journal ISSN

Volume Title

Publisher

Elsevier

Abstract

Using a carbonic anhydrase assay based on membrane inlet mass spectrometry (MIMS), we have extended our earlier investigations of Photosystem II (PSII)-associated carbonic anhydrase activity in spinach PSII preparations (W. Hillier, I. McConnell, M. R. Badger, A. Boussac, V.V. Klimov G. C. Dismukes, T. Wydrzynski Biochemistry 2006, 45:2094). The relationship between the carbonic anhydrase activity and O2 evolution has been evaluated in terms of the effects of metal ion addition, preparation type, light, and response to specific inhibitors. The results indicate that the PSII-associated carbonic anhydrase activity is variable and appears not to be associated specifically with the oxygen evolving activity nor the 33 kDa extrinsic manganese stabilising protein.

Description

Keywords

Keywords: addition reaction; article; enzyme activity; enzyme inhibition; enzyme specificity; mass spectrometry; membrane inlet mass spectrometry; metal binding; nonhuman; oxygen transport; photosystem II; priority journal; protein stability; quantitative analysis; Bicarbonate; Carbonic anhydrase; Manganese stabilising protein; Oxygen evolving complex; Photosystem II

Citation

URI

http://hdl.handle.net/1885/50055

Collections

ANU Research Publications

Source

Biochimica et Biophysica Acta: International journal of Biochemistry and Biophysics

Type

Journal article

Book Title

Entity type

Access Statement

License Rights

DOI

10.1016/j.bbabio.2007.01.019

Restricted until

2037-12-31

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Description
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