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Comparison of telemetric and tail-cuff blood pressure monitoring in adrenocorticotrophic hormone-treated rats

Date

2001

Authors

Fraser, Tafline
Turner, Steven
Mangos, George
Ludbrook, John
Whitworth, Judith

Journal Title

Journal ISSN

Volume Title

Publisher

Blackwell Science Asia

Abstract

1. The aim of the present study was to validate a telemetric blood pressure (BP) monitoring system against tail-cuff blood pressure in both adrenocorticotrophic hormone (ACTH)- and sham-treated rats. In the statistical analyses, we first tested whether there was a detectable effect on systolic blood pressure (SBP) of 10 days treatment with ACTH compared with saline. Second, we compared results of telemetered and tail-cuff measurements and, third, we developed a novel method for estimating the relative power of the two techniques. 2. Twenty-three male Sprague-Dawley rats were randomly divided into two groups: (i) ACTH (100 μg/kg per day, s.c.; n=12) treated; or (ii) sham treated (0.9% saline, s.c.; n=11). Systolic BP was measured by the telemetric system (sampled for 10 s every 2 min) continuously for 4 h (n=16) or for 30 min (n=23) and also by the indirect tail-cuff method daily (n=23). Data were compared within and between groups; ordinary least products (OLP) regression analysis was then performed to test for bias between the two methods. Sample size/power estimations were also performed. 3. Adrenocorticotrophic hormone treatment raised telemetered SBP by 11 mmHg (P<0.001) compared with 14 mmHg (P<0.001) using the tail-cuff method. There was no fixed or proportional bias between the two methods of measurement, as shown by regression analysis. Power calculations indicate that a minimum sample size of six gives a power of telemetered to tail-cuff of 0.84/0.86=0.98. The power of 4 h versus 30 min BP measurements was 0.99/0.82=1.2. 4. Telemetry gave very similar results to the tail-cuff method. Telemetry allows for a longer period of measurement, giving greater power to the study so that fewer animals are needed.

Description

Keywords

Keywords: corticotropin; sodium chloride; animal experiment; animal model; article; blood pressure monitoring; controlled study; hypertension; intermethod comparison; male; nonhuman; rat; regression analysis; sphygmomanometry; systolic blood pressure; telemetry; va Adrenocorticotrophic hormone; Blood pressure; Experimental hypertension; Rats; Telemetry

Citation

Source

Clinical and Experimental Pharmacology and Physiology

Type

Journal article

Book Title

Entity type

Access Statement

License Rights

DOI

10.1046/j.1440-1681.2001.03531.x

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