RNA sequencing of all transcripts and how islet β-cells fail
Date
2014
Authors
Nolan, Christopher
Delghingaro-Augusto, Viviane
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Volume Title
Publisher
American Diabetes Association
Abstract
In this issue, Cnop et al. (1) report on using RNA-sequencing methodology and the response of the whole transcriptome of human islets to 48-h exposure to saturated free fatty acid (FFA) palmitate. They demonstrated that palmitate altered the expression of 1,325 genes and shifted alternate splicing of 3,525 transcripts. This follows on from a similar study by
the same group on the effects on human islets of 48-h
exposure to the proinflammatory cytokines interleukin-1b
and interferon-g in which 3,065 (16%) of transcripts were
modified and, again, alternate splicing of transcripts was
commonly seen (2). However, islet b-cell failure causing diabetes, particularly type 2 diabetes, develops over years and not 48 h. So how do these technically remarkable in vitro experiments on human islets help us to understand islet b-cell failure?
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Diabetes
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Journal article
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2037-12-31
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