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Evaluation of Innate Lymphoid Cells and Dendritic Cells Following Viral Vector Vaccination

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dc.contributor.authorLi, Jerry
dc.contributor.authorRoy, Sreeja
dc.contributor.authorRanasinghe, Charani
dc.contributor.editorBrun, Alejandro
dc.date.accessioned2024-03-11T22:30:51Z
dc.date.issued2022
dc.date.updated2022-10-16T07:27:34Z
dc.description.abstractRecently, we have shown that fate of a vaccine is determined by the cytokine milieu in the innate immune compartment at the early stage of vaccination. Specifically, 24 h post-delivery, level of innate lymphoid cell type 2 (ILC2)-derived IL-13/IL-13Rα2 are the master regulators of DC and also different ILC subsets responsible for modulating the downstream immune outcomes. Here, we provide step-by-step details how to assess different ILC and DC subsets in lung and muscle following intranasal and intramuscular viral vector vaccination, respectively, using multi-color flow cytometry and confocal microscopy.en_AU
dc.format.mimetypeapplication/pdfen_AU
dc.identifier.isbn978-1-0716-2168-4en_AU
dc.identifier.urihttp://hdl.handle.net/1885/315868
dc.language.isoen_AUen_AU
dc.publisherSpringer Verlagen_AU
dc.relation.ispartofVaccine Technologies for Veterinary Viral Diseasesen_AU
dc.relation.isversionof1 Edition
dc.rights© The Author(s), under exclusive license to Springer Science+Business Media. LLC, part of Springer Nature 2022en_AU
dc.titleEvaluation of Innate Lymphoid Cells and Dendritic Cells Following Viral Vector Vaccinationen_AU
dc.typeBook chapteren_AU
local.bibliographicCitation.lastpage153en_AU
local.bibliographicCitation.placeofpublicationNew York, NY
local.bibliographicCitation.startpage137en_AU
local.contributor.affiliationLi, Jerry, College of Health and Medicine, ANUen_AU
local.contributor.affiliationRoy, Sreeja, College of Health and Medicine, ANUen_AU
local.contributor.affiliationRanasinghe, Charani, College of Health and Medicine, ANUen_AU
local.contributor.authoruidLi, Jerry, u5087667en_AU
local.contributor.authoruidRoy, Sreeja, u4383446en_AU
local.contributor.authoruidRanasinghe, Charani, u4107621en_AU
local.description.embargo2099-12-31
local.description.notesImported from ARIESen_AU
local.description.refereedYes
local.identifier.absfor320499 - Immunology not elsewhere classifieden_AU
local.identifier.absfor320705 - Medical virologyen_AU
local.identifier.ariespublicationa383154xPUB26421en_AU
local.identifier.doi10.1007/978-1-0716-2168-4_8en_AU
local.identifier.scopusID2-s2.0-85123973598
local.publisher.urlhttps://link.springer.com/en_AU
local.type.statusPublished Versionen_AU

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