Engineering the male-specificity of Fab against SDM antigen by chain shuffling

Wang, Naidong; Yuan, Anwen; Deng, Zhibang; Yang, Qing; Ma, Jun; Tan, Qinghui; Zhang, Sizhe; Xue, Liqun; Cui, Shuliang

Engineering the male-specificity of Fab against SDM antigen by chain shuffling

Date

2013

Authors

Wang, Naidong

Yuan, Anwen

Deng, Zhibang

Yang, Qing

Ma, Jun

Tan, Qinghui

Zhang, Sizhe

Xue, Liqun

Cui, Shuliang

Publisher

Elsevier BV

Abstract

High-titer serologically detected male (SDM) antibody fragments are essential for specific binding to the SDM antigen and promoting its application. The A8 clone previously obtained from an original phage antibody library was further affinity-matured by l