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Expression Profiling of Herpesvirus and Vaccinia Virus Proteins Using a High-Throughput Baculovirus Screening System

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Date

2005

Authors

Gao, Mian
Brufatto, Nicole
Chen, Tricia
Murley, Laura
Thalakada, Rosanne
Domagala, Megan
Beattie, Bryan
Mamelak, Daniel
Athanasopoulos, Vicki
Johnson, David

Journal Title

Journal ISSN

Volume Title

Publisher

American Chemical Society

Abstract

We have developed a high-throughput system for generating baculoviruses and testing the expression, solubility, and affinity column purification of encoded proteins. We have used this system to generate baculoviruses for and analyze the expression of 337

Description

Keywords

Keywords: complementary DNA; virus protein; amino acid sequence; article; Baculovirus; Cytomegalovirus; Epstein Barr virus; Escherichia coli; Herpes simplex virus 1; Herpes virus; high throughput screening; matrix assisted laser desorption ionization time of flight Baculovirus; Cytomegalovirus; Epstein-Barr virus; Herpes simplex virus; High-throughput protein expression; Vaccinia

Citation

URI

http://hdl.handle.net/1885/84223

Collections

ANU Research Publications

Source

Journal of Proteome Research

Type

Journal article

Book Title

Entity type

Access Statement

License Rights

DOI

10.1021/pr050137u

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