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Thiol-ene reaction: a versatile tool in site-specific labelling of proteins with chemically inert tags for paramagnetic NMR

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Date

2012

Authors

Li, Qing-Feng
Yang, Yin
Maleckis, Ansis
Su, Xun-Cheng
Otting, Gottfried

Journal Title

Journal ISSN

Volume Title

Publisher

Royal Society of Chemistry

Abstract

Site-specific tagging of proteins with paramagnetic lanthanides generates valuable long-range structure restraints for structural biology by NMR spectroscopy. We show that the thiol-ene addition reaction offers a powerful tool for tagging proteins in a chemically stable manner with very small lanthanide tags. This journal is

Description

Keywords

Keywords: cysteine; dipicolinic acid; iodoacetamide; lanthanide; maleimide derivative; oxygen; peptidylprolyl isomerase; protein tag; sulfide; ubiquitin; addition reaction; alpha helix; amino acid sequence; article; chirality; crystal structure; heteronuclear singl

Citation

URI

http://hdl.handle.net/1885/60820

Collections

ANU Research Publications

Source

Chemical Communications

Type

Journal article

Book Title

Entity type

Access Statement

License Rights

DOI

10.1039/c2cc17900h

Restricted until

2037-12-31

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01_Li_Thiol-ene_reaction:_a_2012.pdf (1.23 MB)

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