The Role of Homology and Orthology in the Phylogenomic Analysis of Metazoan Gene Content

Date

2019-01-28

Authors

Pett, Walker
Adamski, Marcin
Adamska, Maja
Francis, Warren R.
Eitel, Michael
Pisani, Davide
Worheide, Gert

Journal Title

Journal ISSN

Volume Title

Publisher

Society for Molecular Biology Evolution

Abstract

Resolving the relationships of animals (Metazoa) is crucial to our understanding of the origin of key traits such as muscles, guts, and nerves. However, a broadly accepted metazoan consensus phylogeny has yet to emerge. In part, this is because the genomes of deeply diverging and fast-evolving lineages may undergo significant gene turnover, reducing the number of orthologs shared with related phyla. This can limit the usefulness of traditional phylogenetic methods that rely on alignments of orthologous sequences. Phylogenetic analysis of gene content has the potential to circumvent this orthology requirement, with binary presence/absence of homologous gene families representing a source of phylogenetically informative characters. Applying binary substitution models to the gene content of 26 complete animal genomes, we demonstrate that patterns of gene conservation differ markedly depending on whether gene families are defined by orthology or homology, that is, whether paralogs are excluded or included. We conclude that the placement of some deeply diverging lineages may exceed the limit of resolution afforded by the current methods based on comparisons of orthologous protein sequences, and novel approaches are required to fully capture the evolutionary signal from genes within genomes.

Description

Keywords

phylogenomics, gene content, animal phylogeny, homology, orthology

Citation

Source

Molecular Biology and Evolution

Type

Journal article

Book Title

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Access Statement

License Rights

DOI

10.1093/molbev/msz013

Restricted until

2037-12-31