Holographic microscope and its biological application

Date

2021

Authors

Quan, Xiangyu
Kato, Daisuke
Daria, Vincent
Matoba, Osamu
Wake, Hiroaki

Journal Title

Journal ISSN

Volume Title

Publisher

Elsevier

Abstract

Holographic structured illumination combined with optogenetics enables patterned stimulation of neurons and glial cells in an intact living brain. Moreover, in vivo functional imaging of cellular activity with recent advanced microscope technologies allows for visualization of the cellular responses during learning, emotion and cognition. Integrating these techniques can be used to verify the link between cell function and behavior output. However, there are technical limitations to stimulate multiple cells with high spatial and temporal resolution with available techniques of optogenetic stimulation. Here, we summarized a two-photon microscope combined with holographic system to stimulate multiple cells with high spatial and temporal resolution for living mice and their biological application.

Description

Keywords

holographic microscope, two-photon microscope, three-dimensional (3D) light patterns, optogenetics

Citation

Source

Neuroscience Research

Type

Journal article

Book Title

Entity type

Access Statement

Open Access

License Rights

Creative Commons Attribution-NonCommercial-NoDerivatives License

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