Holographic microscope and its biological application
Date
2021
Authors
Quan, Xiangyu
Kato, Daisuke
Daria, Vincent
Matoba, Osamu
Wake, Hiroaki
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
Holographic structured illumination combined with optogenetics enables patterned stimulation of neurons and glial cells in an intact living brain. Moreover, in vivo functional imaging of cellular activity with recent advanced microscope technologies allows for visualization of the cellular responses during learning, emotion and cognition. Integrating these techniques can be used to verify the link between cell function and behavior output. However, there are technical limitations to stimulate multiple cells with high spatial and temporal resolution with available techniques of optogenetic stimulation. Here, we summarized a two-photon microscope combined with holographic system to stimulate multiple cells with high spatial and temporal resolution for living mice and their biological application.
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Keywords
holographic microscope, two-photon microscope, three-dimensional (3D) light patterns, optogenetics
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Source
Neuroscience Research
Type
Journal article
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Access Statement
Open Access
License Rights
Creative Commons Attribution-NonCommercial-NoDerivatives License
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