A generalised module for the selective extracellular accumulation of recombinant proteins

Date

2012

Authors

Sevastsyanovich, Yanina R
Leyton, Denisse
Wells, Timothy J
Wardius, Catherine A
Tveen-Jensen, Karina
Morris, Faye C
Knowles, Timothy J
Cunningham, Adam F
Cole, Jeffrey A
Henderson, Ian R

Journal Title

Journal ISSN

Volume Title

Publisher

BioMed Central

Abstract

Background: It is widely believed that laboratory strains of Escherichia coli, including those used for industrial production of proteins, do not secrete proteins to the extracellular milieu.Results: Here, we report the development of a generalised module, based on an E. coli autotransporter secretion system, for the production of extracellular recombinant proteins. We demonstrate that a wide variety of structurally diverse proteins can be secreted as soluble proteins when linked to the autotransporter module. Yields were comparable to those achieved with other bacterial secretion systems.Conclusions: The advantage of this module is that it relies on a relatively simple and easily manipulated secretion system, exhibits no apparent limitation to the size of the secreted protein and can deliver proteins to the extracellular environment at levels of purity and yields sufficient for many biotechnological applications.

Description

Keywords

Keywords: recombinant protein; recombinant protein; article; bacterial cell; bacterial secretion system; chimera; controlled study; culture medium; Escherichia coli; extracellular space; molecular model; molecular size; nonhuman; polyacrylamide gel electrophoresis; Autotransporter; Escherhichia coli; Recombinant protein production; Secretion

Citation

Source

Microbial Cell Factories

Type

Journal article

Book Title

Entity type

Access Statement

Open Access

License Rights

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