The mitochondrial genome can be altered or lost without lethal effect in the petie-negative yeast Debarymyces (Schwanniomyces) occidentalis

Date

2002

Authors

Fernet, C
Clark-Walker, George
Claisse, M

Journal Title

Journal ISSN

Volume Title

Publisher

Springer

Abstract

The nature of mutations affecting several cytochrome-deficient mutants of Debaryomyces (Schwanniomyces) occidentalis has been characterized. The DR12 mutant, which is deficient in cytochrome b, and the B10Mn mutant, which is deficient in cytochromes b and a, a3, are deleted in the mitochondrial CYB and COX1 genes respectively. The B10 strain, which is partially deficient in cytochrome b, has no detectable change in its mitochondrial DNA and possibly carries nuclear lesion(s). These three mutants, unlike the rho- and rhoo "petite" mutants of Saccharomyces cerevisiae, can still grow on non-fermentable substrates, due to the development of a salicylhydroxamic acid (SHAM)-sensitive alternative pathway linked to phosphorylation at site 1. A gly- mutant lacking mtDNA and respiratory capacity has been isolated. For the first time, it is demonstrated that mtDNA can be altered or even lost without lethal consequence in D. occidentalis, although this yeast was classified as a petite-negative species.

Description

Keywords

Keywords: chloramphenicol; cytochrome a; cytochrome b; erythromycin; mitochondrial DNA; mitochondrial protein; salicylhydroxamic acid; absorption spectroscopy; classification; controlled study; Debaryomyces; Debaryomyces occidentalis; DNA determination; drug effect Debaryomyces (Schwanniomyces) occidentalis; Mitochondrial DNA; Petite-negative

Citation

Source

Current Genetics

Type

Journal article

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2037-12-31