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Megahertz single-particle imaging at the European XFEL

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dc.contributor.authorSobolev, Egor
dc.contributor.authorZolotarev, Sergei
dc.contributor.authorGiewekemeyer, Klaus
dc.contributor.authorBielecki, Johan
dc.contributor.authorOkamoto, Kento
dc.contributor.authorReddy, Hemanth K. N.
dc.contributor.authorAndreasson, Jakob
dc.contributor.authorAyyer, Kartik
dc.contributor.authorBarak, Imrich
dc.contributor.authorBari, Sadia
dc.contributor.authorBarty, A
dc.contributor.authorBean, R
dc.contributor.authorBobkov, Sergey
dc.contributor.authorChapman, Henry N.
dc.contributor.authorChojnowski, Grzegorz
dc.contributor.authorDaurer, Benedikt J.
dc.contributor.authorRode, Andrei
dc.date.accessioned2022-07-20T03:28:39Z
dc.date.available2022-07-20T03:28:39Z
dc.date.issued2020
dc.date.updated2021-08-01T08:23:17Z
dc.description.abstractThe emergence of high repetition-rate X-ray free-electron lasers (XFELs) powered by superconducting accelerator technology enables the measurement of significantly more experimental data per day than was previously possible. The European XFEL is expected to provide 27,000 pulses per second, over two orders of magnitude more than any other XFEL. The increased pulse rate is a key enabling factor for single-particle X-ray diffractive imaging, which relies on averaging the weak diffraction signal from single biological particles. Taking full advantage of this new capability requires that all experimental steps, from sample preparation and delivery to the acquisition of diffraction patterns, are compatible with the increased pulse repetition rate. Here, we show that single-particle imaging can be performed using X-ray pulses at megahertz repetition rates. The results obtained pave the way towards exploiting high repetition-rate X-ray free-electron lasers for single-particle imaging at their full repetition rate.en_AU
dc.description.sponsorshipWe acknowledge European XFEL in Schenefeld, Germany, for provision of X-ray free-electron laser beamtime at Scientific Instrument SPB/SFX and would like to thank the instrument group and facility staff for their assistance. We acknowledge the use of the XBI biological sample preparation laboratory, enabled by the XBI User Consortium. The results of the work were obtained using Maxwell computational resources operated at Deutsches Elektronen-Synchrotron (DESY), Hamburg, Germany, and computational resources of MCC NRC “Kurchatov Institute.” This research used resources of the National Synchrotron Light Source II, a U.S. Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Brookhaven National Laboratory under Contract No. DE-SC0012704. We acknowledge the support of funding from: the Swedish Foundation for International Cooperation in Research and Higher Education (STINT); Helmholtz Associations Initiative and Networking Fund and the Russian Science Foundation grant HRSF-0002/18-41-0600; the Russian Science Foundation grant 18-14-00321; European Research Council, “Frontiers in Attosecond X-ray Science: Imaging and Spectroscopy (AXSIS),” ERC-2013-SyG 609920; Fellowship from the Joachim Herz Stiftung; Singapore National Research Foundation Grant number NRF-CRP16-2015-05; Ministry of Education, Science, Research and Sport of the Slovak Republic and by grant APVV-18-0104; the project CZ.02.1.01/0.0/0.0/16_019/0000789 (ADONIS) from European Regional Development Fund, Chalmers Area of Advance; Material Science and the Ministry of Education, Youth and Sports as part of targeted support from the National Programme of Sustainability II; US National Science Foundation (NSF) Science and Technology Center BioXFEL Award 1231306; Helmholtz Initiative and Networking Fund through the Young Investigators Group Program and Deutsche Forschungsgemeinschaft, project B03/SFB755; VR starting grant (2018-03387); FORMAS future research leader (2018-00421); KVA Biosciences 2018 (BS2018-0053); NSF 1231306; German Ministry for Education and Research, BMBF (grant No. 05K2016—Visavix); the Heinrich Pette Institute, Leibniz Institute for Experimental Virology is supported by the Free and Hanseatic City of Hamburg and the Federal Ministry of Health; NSF STC BioXFEL grant 1231306; The National Research Foundation (NRF) of Korea (Grant No. 2017K1A3A7A09016380); the Röntgen-Ångström Cluster; the Swedish Research Council; the Swedish Foundation for Strategic Research. We thank Arwen Pearson for critical reading of the manuscript. Open access funding provided by Uppsala University.en_AU
dc.format.mimetypeapplication/pdfen_AU
dc.identifier.issn2399-3650en_AU
dc.identifier.urihttp://hdl.handle.net/1885/269813
dc.language.isoen_AUen_AU
dc.provenanceThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.en_AU
dc.publisherNature Publishing Groupen_AU
dc.rights© 2020 The authorsen_AU
dc.rights.licenseCreative Commonsen_AU
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_AU
dc.sourceCommunications Physicsen_AU
dc.titleMegahertz single-particle imaging at the European XFELen_AU
dc.typeJournal articleen_AU
dcterms.accessRightsOpen Accessen_AU
local.bibliographicCitation.issue1en_AU
local.bibliographicCitation.lastpage11en_AU
local.bibliographicCitation.startpage1en_AU
local.contributor.affiliationSobolev, Egor, European Molecular Biology Laboratoryen_AU
local.contributor.affiliationZolotarev, Sergei, Kurchatov Instituteen_AU
local.contributor.affiliationGiewekemeyer, Klaus, European XFEL GmbHen_AU
local.contributor.affiliationBielecki, Johan, European XFEL GmbHen_AU
local.contributor.affiliationOkamoto, Kento, Uppsala Universityen_AU
local.contributor.affiliationReddy, Hemanth K. N., Uppsala Universityen_AU
local.contributor.affiliationAndreasson, Jakob, Academy of Sciences of the Czech Republicen_AU
local.contributor.affiliationAyyer, Kartik, Center for Free-Electron Laser Science, DESYen_AU
local.contributor.affiliationBarak, Imrich, Institute of Molecular Biologyen_AU
local.contributor.affiliationBari, Sadia, Deutsches Elektronen-Synchrotron DESYen_AU
local.contributor.affiliationBarty, A, Center for Free-Electron Laser Scienceen_AU
local.contributor.affiliationBean, R, European XFEL GmbHen_AU
local.contributor.affiliationBobkov, Sergey, Kurchatov Instituteen_AU
local.contributor.affiliationChapman, Henry N., Deutsches Elektronen-Synchrotron DESYen_AU
local.contributor.affiliationChojnowski, Grzegorz , European Molecular Biology Laboratory, c/o DESYen_AU
local.contributor.affiliationDaurer, Benedikt J. , Uppsala Universityen_AU
local.contributor.affiliationRode, Andrei, College of Science, ANUen_AU
local.contributor.authoruidRode, Andrei, u8913168en_AU
local.description.notesImported from ARIESen_AU
local.identifier.absfor000000 - Internal ANU use onlyen_AU
local.identifier.ariespublicationa383154xPUB14328en_AU
local.identifier.citationvolume3en_AU
local.identifier.doi10.1038/s42005-020-0362-yen_AU
local.publisher.urlhttps://www.nature.com/en_AU
local.type.statusPublished Versionen_AU

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