Imaging pluripotent cell migration in Drosophila

dc.contributor.authorMurray, Michael
dc.contributor.authorSaint, Robert
dc.date.accessioned2015-12-10T22:18:19Z
dc.date.issued2011
dc.date.updated2016-02-24T11:41:37Z
dc.description.abstractDrosophila melanogaster offers a powerful system for the analysis of cell migration. In the embryo, -pluripotent cells of the mesodermal and endodermal primordia undergo epithelial-mesenchymal transitions and cell migration, while primordial germ cells migrate through an endodermal barrier to form the gonads. Visualisation of these migrations has traditionally been achieved by staining fixed embryos at different developmental stages or through live imaging of cells using tissue-specific expression of marker fluorescent proteins. More recently, photoactivatable fluorescence proteins have allowed the labelling of small groups of cells or single cells so that their migratory patterns and fate can be followed. By fusing the photoactivatable fluorescent protein to proteins that mark different subcellular components, it is now possible to visualise different aspects of the cells as they migrate. Here, we review previous studies of the migration of pluripotent embryonic cells and describe, in detail, methods for visualising these cells.
dc.identifier.isbn9781617791444
dc.identifier.urihttp://hdl.handle.net/1885/51369
dc.publisherHumana Press Inc.
dc.relation.ispartofStem Cell Migration: Methods and Protocols
dc.subjectKeywords: fixative; green fluorescent protein; animal; animal embryo; article; cell motion; chemistry; confocal microscopy; cytology; Drosophila melanogaster; epithelial mesenchymal transition; fluorescence; gene expression regulation; genetics; germ cell; gonad; i
dc.titleImaging pluripotent cell migration in Drosophila
dc.typeBook chapter
local.bibliographicCitation.lastpage184
local.bibliographicCitation.placeofpublicationNew York
local.bibliographicCitation.startpage169
local.contributor.affiliationMurray, Michael, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationSaint, Robert, College of Medicine, Biology and Environment, ANU
local.contributor.authoremailrepository.admin@anu.edu.au
local.contributor.authoruidMurray, Michael, u4055137
local.contributor.authoruidSaint, Robert, u4042812
local.description.embargo2037-12-31
local.description.notesImported from ARIES
local.identifier.absfor060103 - Cell Development, Proliferation and Death
local.identifier.absfor060106 - Cellular Interactions (incl. Adhesion, Matrix, Cell Wall)
local.identifier.absseo929999 - Health not elsewhere classified
local.identifier.ariespublicationu8611701xPUB222
local.identifier.doi10.1007/978-1-61779-145-1_12
local.identifier.scopusID2-s2.0-80052609713
local.identifier.uidSubmittedByu8611701
local.type.statusPublished Version

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