Preparation of Label from Small Cell Numbers for Microarray Screening

Wilson, Heather; O'Neill, Helen

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Preparation of Label from Small Cell Numbers for Microarray Screening

Date

2004

Authors

Wilson, Heather

O'Neill, Helen

Publisher

Mary Ann Liebert Inc.

Abstract

Common methods for amplification of labelled cRNA for hybridisation to Affymetrix GeneChips (Affymetrix Inc., Santa Clara, CA) assume that starting material is not limiting and require 2-5 μg of total RNA. However, often the target population of cells un