Improving focal photostimulation of cortical neurons with pre-derived wavefront correction
Date
2017
Authors
Choy, Julian
Sane, Sharmila
Lee, W. M. Steve
Stricker, Christian
Bachor, Hans
Daria, Vincent
Journal Title
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Volume Title
Publisher
Frontiers Research Foundation
Abstract
Recent progress in neuroscience to image and investigate brain function has been made possible
by impressive developments in optogenetic and opto-molecular tools. Such research requires advances
in optical techniques for the delivery of light through brain tissue with high spatial resolution.
The tissue causes distortions to the wavefront of the incoming light which broadens the focus and
consequently reduces the intensity and degrades the resolution. Such effects are detrimental in
techniques requiring focal stimulation. Adaptive wavefront correction has been demonstrated to
compensate for these distortions. However, iterative derivation of the corrective wavefront
introduces time constraints that limit its applicability to probe living cells. Here, we
demonstrate that we can pre-determine and generalize a small set of Zernike modes to correct for
aberrations of the light propagating through specific brain regions. A priori identification of a
corrective wavefront is a direct and fast technique that improves the quality of the focus without
the need for iterative adaptive wavefront correction. We verify our technique by measuring the
efficiency of two-photon photolysis of caged neurotransmitters along the dendrites of a whole-cell
patched neuron. Our results show that encoding the selected Zernike modes on the excitation ight
can improve light propagation through brain slices of rats as observed by the neuron’s evoked
excitatory post-synaptic potential in response to localized focal uncaging at the spines of the
neuron’s dendrites.
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Keywords
adaptive optics, two photon microscopy, spatial light modulator, two-photon photolysis
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Source
Frontiers in Cellular Neuroscience
Type
Journal article
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Open Access
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Creative Commons Attribution License (CC BY)
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