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Xenopus laevis Oocytes

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Date

2010

Authors

Broer, Stefan

Journal Title

Journal ISSN

Volume Title

Publisher

Humana Press Inc.

Abstract

Xenopus oocytes are a versatile expression system particularly suited for membrane transporters and channels. Oocytes have little background activity and therefore offer a very high signal-to-noise ratio for transporter and channel characterization. This chapter provides an overview of the basic methods used for the analysis of membrane transporters in this system, including preparation of oocytes, assays of transport activity, protocols for immunostaining and fluorescence microscopy, and other assays to study surface expression.

Description

Keywords

Two-electrode voltage clamp, surface biotinylation, flux assay, transport, microtransplantation, efflux, ion-sensitive electrodes

Citation

URI

http://hdl.handle.net/1885/258587

Collections

ANU Research Publications

Source

Type

Book chapter

Book Title

Membrane Transporters in Drug Discovery and Development; methods and protocols (2nd ed)

Entity type

Access Statement

License Rights

DOI

10.1007/978-1-60761-700-6_16

Restricted until

2099-12-31

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Description
01_Broer_Xenopus_laevis_Oocytes_2010.pdf (426.91 KB)

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