MICROTUBULE ORGANIZATION 1 Regulates Structure and Function of Microtubule Arrays during Mitosis and Cytokinesis in the Arabidopsis Root

Date

2006

Authors

Kawamura, Eiko
Himmelspach, Regina
Rashbrooke, Madeleine
Whittington, A
Gale, Kevin R
Collings, David
Wasteneys, Geoffrey

Journal Title

Journal ISSN

Volume Title

Publisher

American Society of Plant Biologists

Abstract

MICROTUBULE ORGANIZATION 1 (MOR1) is a plant member of the highly conserved MAP215/Dis1 family of microtubule-associated proteins. Prior studies with the temperature-sensitive mor1 mutants of Arabidopsis (Ambidopsis thaliana), which harbor single amino acid substitutions in an N-terminal HEAT repeat, proved that MOR1 regulates cortical microtubule organization and function. Here we demonstrate by use of live cell imaging and immunolabeling that the mor1-1 mutation generates specific defects in the microtubule arrays of dividing vegetative cells. Unlike the universal cortical microtubule disorganization in elongating mor1-1 cells, disruption of mitotic and cytokinetic microtubule arrays was not detected in all dividing cells. Nevertheless, quantitative analysis identified distinct defects in preprophase bands (PPBs), spindles, and phragmoplasts. In nearly one-half of dividing cells at the restrictive temperature of 30°C, PPBs were not detected prior to spindle formation, and those that did form were often disrupted, mor1-1 spindles and phragmoplasts were short and abnormally organized and persisted for longer times than in wild-type cells. The reduced length of these arrays predicts that the component microtubule lengths are also reduced, suggesting that microtubule length is a critical determinant of spindle and phragmoplast structure, orientation, and function. Microtubule organizational defects led to aberrant chromosomal arrangements, misaligned or incomplete cell plates, and multinucleate cells. Antiserum raised against an N-terminal MOR1 sequence labeled the full length of microtubules in interphase arrays, PPBs, spindles, and phragmoplasts. Continued immunolabeling of the disorganized and short microtubules of mor1-1 at the restrictive temperature demonstrated that the mutant mor1-1L174F protein loses function without dissociating from microtubules, providing important insight into the mechanism by which MOR1 may regulate microtubule length.

Description

Keywords

Keywords: Amino acids; Proteins; Cell imaging; Cytokinesis; Microtubule arrays; Phragmoplasts; Plant cell culture; Amino Acids; Cell Division; Cells; Proteins; Arabidopsis; Arabidopsis protein; microtubule associated protein; MOR1 protein, Arabidopsis; Arabidopsis;

Citation

Source

Plant Physiology

Type

Journal article

Book Title

Entity type

Access Statement

License Rights

DOI

10.1104/pp.105.069989

Restricted until

2037-12-31