Spectral reading of optical resonance-encoded cells in microfluidics
dc.contributor.author | Humar, Matjaž | |
dc.contributor.author | Upadhya, Avinash | |
dc.contributor.author | Yun, Seok Hyun | |
dc.date.accessioned | 2020-04-06T01:37:43Z | |
dc.date.issued | 2017-07-07 | |
dc.date.updated | 2019-11-25T07:50:04Z | |
dc.description.abstract | The ability to label individual cells is useful for single-cell-level studies of complex cellular interactions and heterogeneity. Optically readable cell labeling is attractive as it can be investigated non-invasively and repeatedly at high speeds. Here, we demonstrate the feasibility of large-scale cell barcoding and identification using fluorescent polystyrene microbeads loaded into cells. Intracellular beads with different diameters in a range of 5 to 12 μm generate spectrally distinguished features or barcodes. A microfluidic chip was used to measure fluorescence resonance peaks emitted from individual cells. An algorithm comparing the peak wavelengths to a reference barcode library allowed barcode identification with high accuracy. This work provides a guideline to increase the number of unique identifiers and reduce various false-positive and false-negative errors. | en_AU |
dc.description.sponsorship | This research was supported by the U.S. National Institutes of Health (R01-CA192878, P41-EB015903, P01- HL120839, DP1-OD022296), National Science Foundation (CBET-1264356, EEC-1358296, ECCS-1505569, CMMI-1562863), and MGH Research Scholar Award. M.H. was supported in part by the Marie Curie International Outgoing Fellowship N° 627274 within the 7th European Community Framework Programme. | en_AU |
dc.format.extent | 9 pages | en_AU |
dc.format.mimetype | application/pdf | en_AU |
dc.identifier.issn | 1473-0197 | en_AU |
dc.identifier.uri | http://hdl.handle.net/1885/202747 | |
dc.language.iso | en_AU | en_AU |
dc.publisher | Royal Society of Chemistry | en_AU |
dc.rights | © The Royal Society of Chemistry 2017 | en_AU |
dc.source | Lab on a Chip | en_AU |
dc.title | Spectral reading of optical resonance-encoded cells in microfluidics | en_AU |
dc.type | Journal article | en_AU |
dcterms.dateAccepted | 2017-06-28 | |
local.bibliographicCitation.issue | 16 | en_AU |
local.bibliographicCitation.lastpage | 2784 | en_AU |
local.bibliographicCitation.startpage | 2777 | en_AU |
local.contributor.affiliation | Humar, Matjaž, Harvard Medical School | en_AU |
local.contributor.affiliation | Upadhya, Avinash, College of Engineering and Computer Science, The Australian National University | en_AU |
local.contributor.affiliation | Yun, Seok Hyun, Harvard Medical School | en_AU |
local.contributor.authoremail | u5163163@anu.edu.au | en_AU |
local.contributor.authoruid | Upadhya, Avinash, u5163163 | en_AU |
local.description.embargo | 2037-12-31 | |
local.description.notes | Imported from ARIES | en_AU |
local.identifier.absfor | 090399 - Biomedical Engineering not elsewhere classified | en_AU |
local.identifier.absseo | 970106 - Expanding Knowledge in the Biological Sciences | en_AU |
local.identifier.ariespublication | u4485658xPUB1006 | en_AU |
local.identifier.citationvolume | 17 | en_AU |
local.identifier.doi | 10.1039/c7lc00220c | en_AU |
local.identifier.essn | 1473-0189 | en_AU |
local.identifier.scopusID | 2-s2.0-85027179875 | |
local.identifier.thomsonID | 000407168900006 | |
local.identifier.uidSubmittedBy | u4485658 | en_AU |
local.publisher.url | https://www.rsc.org/ | en_AU |
local.type.status | Published Version | en_AU |
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