Spectral reading of optical resonance-encoded cells in microfluidics

dc.contributor.authorHumar, Matjaž
dc.contributor.authorUpadhya, Avinash
dc.contributor.authorYun, Seok Hyun
dc.date.accessioned2020-04-06T01:37:43Z
dc.date.issued2017-07-07
dc.date.updated2019-11-25T07:50:04Z
dc.description.abstractThe ability to label individual cells is useful for single-cell-level studies of complex cellular interactions and heterogeneity. Optically readable cell labeling is attractive as it can be investigated non-invasively and repeatedly at high speeds. Here, we demonstrate the feasibility of large-scale cell barcoding and identification using fluorescent polystyrene microbeads loaded into cells. Intracellular beads with different diameters in a range of 5 to 12 μm generate spectrally distinguished features or barcodes. A microfluidic chip was used to measure fluorescence resonance peaks emitted from individual cells. An algorithm comparing the peak wavelengths to a reference barcode library allowed barcode identification with high accuracy. This work provides a guideline to increase the number of unique identifiers and reduce various false-positive and false-negative errors.en_AU
dc.description.sponsorshipThis research was supported by the U.S. National Institutes of Health (R01-CA192878, P41-EB015903, P01- HL120839, DP1-OD022296), National Science Foundation (CBET-1264356, EEC-1358296, ECCS-1505569, CMMI-1562863), and MGH Research Scholar Award. M.H. was supported in part by the Marie Curie International Outgoing Fellowship N° 627274 within the 7th European Community Framework Programme.en_AU
dc.format.extent9 pagesen_AU
dc.format.mimetypeapplication/pdfen_AU
dc.identifier.issn1473-0197en_AU
dc.identifier.urihttp://hdl.handle.net/1885/202747
dc.language.isoen_AUen_AU
dc.publisherRoyal Society of Chemistryen_AU
dc.rights© The Royal Society of Chemistry 2017en_AU
dc.sourceLab on a Chipen_AU
dc.titleSpectral reading of optical resonance-encoded cells in microfluidicsen_AU
dc.typeJournal articleen_AU
dcterms.dateAccepted2017-06-28
local.bibliographicCitation.issue16en_AU
local.bibliographicCitation.lastpage2784en_AU
local.bibliographicCitation.startpage2777en_AU
local.contributor.affiliationHumar, Matjaž, Harvard Medical Schoolen_AU
local.contributor.affiliationUpadhya, Avinash, College of Engineering and Computer Science, The Australian National Universityen_AU
local.contributor.affiliationYun, Seok Hyun, Harvard Medical Schoolen_AU
local.contributor.authoremailu5163163@anu.edu.auen_AU
local.contributor.authoruidUpadhya, Avinash, u5163163en_AU
local.description.embargo2037-12-31
local.description.notesImported from ARIESen_AU
local.identifier.absfor090399 - Biomedical Engineering not elsewhere classifieden_AU
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciencesen_AU
local.identifier.ariespublicationu4485658xPUB1006en_AU
local.identifier.citationvolume17en_AU
local.identifier.doi10.1039/c7lc00220cen_AU
local.identifier.essn1473-0189en_AU
local.identifier.scopusID2-s2.0-85027179875
local.identifier.thomsonID000407168900006
local.identifier.uidSubmittedByu4485658en_AU
local.publisher.urlhttps://www.rsc.org/en_AU
local.type.statusPublished Versionen_AU

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