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A rapid and versatile combined DNA/RNA extraction protocol and its application to the analysis of a novel DNA marker set polymorphic between Arabidopsis thaliana ecotypes Col-0 and Landsberg erecta

Berendzen, Kenneth; Searle, Iain; Ravenscroft, Dean; Koncz, Csaba; Batschauer, Alfred; Coupland, George; Somssich, Imre E.; Ülker, Bekir

Description

BACKGROUND Many established PCR-based approaches in plant molecular biology rely on lengthy and expensive methods for isolation of nucleic acids. Although several rapid DNA isolation protocols are available, they have not been tested for simultaneous RNA isolation for RT-PCR applications. In addition, traditional map-based cloning technologies often use ill-proportioned marker regions even when working with the model plant Arabidopsis thaliana, where the availability of the full genome sequence...[Show more]

CollectionsANU Research Publications
Date published: 2005-08-23
Type: Journal article
URI: http://hdl.handle.net/1885/95278
Source: Plant Methods
DOI: 10.1186/1746-4811-1-4

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