Berendzen, Kenneth; Searle, Iain; Ravenscroft, Dean; Koncz, Csaba; Batschauer, Alfred; Coupland, George; Somssich, Imre E.; Ülker, Bekir
BACKGROUND Many established PCR-based approaches in plant molecular biology rely on lengthy and expensive methods for isolation of nucleic acids. Although several rapid DNA isolation protocols are available, they have not been tested for simultaneous RNA isolation for RT-PCR applications. In addition, traditional map-based cloning technologies often use ill-proportioned marker regions even when working with the model plant Arabidopsis thaliana, where the availability of the full genome sequence...[Show more]
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