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Identification of an ATP-binding cassette transporter involved in bicarbonate uptake in the cyanobacterium Synechococcus sp. strain PCC7942

Omata, Tatsuo; Price, Graeme (Dean); Badger, Murray; Okamura, Masato; Gohta, Satoshi; Ogawa, Teruo

Description

Exposure of cells of cyanobacteria (blue-green algae) grown under high- CO2 conditions to inorganic C-limitation induces transcription of particular genes and expression of high-affinity CO2 and HCO3- transport systems. Among the low-CO2-inducible transcription units of Synechococcus sp. strain PCC 7942 is the cmpABCD operon, encoding an ATP-binding cassette transporter similar to the nitrate/nitrite transporter of the same cyanobacterium. A nitrogen-regulated promoter was used to selectively...[Show more]

dc.contributor.authorOmata, Tatsuo
dc.contributor.authorPrice, Graeme (Dean)
dc.contributor.authorBadger, Murray
dc.contributor.authorOkamura, Masato
dc.contributor.authorGohta, Satoshi
dc.contributor.authorOgawa, Teruo
dc.date.accessioned2015-12-13T23:40:40Z
dc.identifier.issn0027-8424
dc.identifier.urihttp://hdl.handle.net/1885/94571
dc.description.abstractExposure of cells of cyanobacteria (blue-green algae) grown under high- CO2 conditions to inorganic C-limitation induces transcription of particular genes and expression of high-affinity CO2 and HCO3- transport systems. Among the low-CO2-inducible transcription units of Synechococcus sp. strain PCC 7942 is the cmpABCD operon, encoding an ATP-binding cassette transporter similar to the nitrate/nitrite transporter of the same cyanobacterium. A nitrogen-regulated promoter was used to selectively induce expression of the cmpABCD genes by growth of transgenic cells on nitrate under high CO2 conditions. Measurements of the initial rate of HCO3- uptake after onset of light, and of the steady-state rate of HCO3- uptake in the light, showed that the controlled induction of the cmp genes resulted in selective expression of high-affinity HCO3- transport activity. The forced expression of cmpABCD did not significantly increase the CO2 uptake capabilities of the cells. These findings demonstrated that the cmpABCD genes encode a high- affinity HCO3- transporter. A deletion mutant of cmpAB (M42) retained low CO2-inducible activity of HCO3- transport, indicating the occurrence of HCO3- transporter(s) distinct from the one encoded by cmpABCD. HCO3- uptake by low-CO2-induced M42 cells showed lower affinity for external HCO3- than for wild-type cells under the same conditions, showing that the HCO3- transporter encoded by cmpABCD has the highest affinity for HCO3- among the HCO3- transporters present in the cyanobacterium. This appears to be the first unambiguous identification and description of a primary active HCO3- transporter.
dc.publisherNational Academy of Sciences (USA)
dc.sourcePNAS - Proceedings of the National Academy of Sciences of the United States of America
dc.subjectKeywords: adenosine triphosphate; bicarbonate; article; bacterial growth; carbon dioxide fixation; cyanobacterium; genetic analysis; gram negative bacterium; nonhuman; photosynthesis; priority journal; ATP-Binding Cassette Transporters; Bicarbonates; Cyanobacteria;
dc.titleIdentification of an ATP-binding cassette transporter involved in bicarbonate uptake in the cyanobacterium Synechococcus sp. strain PCC7942
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume96
dc.date.issued1999
local.identifier.absfor060501 - Bacteriology
local.identifier.ariespublicationMigratedxPub24180
local.type.statusPublished Version
local.contributor.affiliationOmata, Tatsuo, Nagoya University
local.contributor.affiliationPrice, Graeme (Dean), College of Medicine, Biology and Environment, ANU
local.contributor.affiliationBadger, Murray, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationOkamura, Masato, Nagoya University
local.contributor.affiliationGohta, Satoshi, Nagoya University
local.contributor.affiliationOgawa, Teruo, Nagoya University
local.description.embargo2037-12-31
local.bibliographicCitation.issue23
local.bibliographicCitation.startpage13571
local.bibliographicCitation.lastpage13576
local.identifier.doi10.1073/pnas.96.23.13571
dc.date.updated2015-12-12T09:30:01Z
local.identifier.scopusID2-s2.0-0033539480
CollectionsANU Research Publications

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