Skip navigation
Skip navigation
A change is coming. Click to see a sneak peek of the new Open Research Repository.

Dealing with intractable protein cores: protein sequencing of the Mcg IgG and the Yvo IgM heavy chain variable domains

link to publisher version

  • Altmetric Citations

Shaw, Denis; Shultz, Brandon; Ramsland, Paul; Edmundson, Allen

Description

The VH domains of two human monoclonal antibodies, designated Mcg IgGI(λ) and Yvo IgM(κ), were particularly intractable to standard protein sequencing protocols. Peptides liberated from the VH domains of these proteins, using standard enzymatic or chemi

dc.contributor.authorShaw, Denis
dc.contributor.authorShultz, Brandon
dc.contributor.authorRamsland, Paul
dc.contributor.authorEdmundson, Allen
dc.date.accessioned2015-12-13T23:40:05Z
dc.date.available2015-12-13T23:40:05Z
dc.identifier.issn0952-3499
dc.identifier.urihttp://hdl.handle.net/1885/94297
dc.description.abstractThe VH domains of two human monoclonal antibodies, designated Mcg IgGI(λ) and Yvo IgM(κ), were particularly intractable to standard protein sequencing protocols. Peptides liberated from the VH domains of these proteins, using standard enzymatic or chemi
dc.publisherJohn Wiley & Sons Inc
dc.sourceJournal of Molecular Recognition
dc.subjectKeywords: dodecyl sulfate sodium; immunoglobulin G; immunoglobulin heavy chain; monoclonal antibody; Bence Jones protein; immunoglobulin; immunoglobulin M; myeloma immunoglobulins; paraprotein; amino acid sequence; polyacrylamide gel electrophoresis; protein degrad Antibody crystallography; Human monoclonal antibodies; Insoluble peptides; Protein sequencing
dc.titleDealing with intractable protein cores: protein sequencing of the Mcg IgG and the Yvo IgM heavy chain variable domains
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume15
dc.date.issued2002
local.identifier.absfor110106 - Medical Biochemistry: Proteins and Peptides (incl. Medical Proteomics)
local.identifier.ariespublicationMigratedxPub23857
local.type.statusPublished Version
local.contributor.affiliationShaw, Denis, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationShultz, Brandon, Oklahoma Medical Research Foundation
local.contributor.affiliationRamsland, Paul, Austin Research Institute
local.contributor.affiliationEdmundson, Allen, Oklahoma Medical Research Foundation
local.bibliographicCitation.startpage341
local.bibliographicCitation.lastpage348
local.identifier.doi10.1002/jmr.596
dc.date.updated2015-12-12T09:27:44Z
local.identifier.scopusID2-s2.0-0036761294
CollectionsANU Research Publications

Download

There are no files associated with this item.
Show simple item record


Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  17 November 2022/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator